Related Experiment Video
Updated: Aug 8, 2025

14:53
Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
11.3K
Exploiting Non-Conventional Yeasts for Low-Alcohol Beer Production
João Simões1,2, Eduardo Coelho1,2, Paulo Magalhães3
1CEB-Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
Microorganisms
|February 25, 2023
Summary
Non-Saccharomyces yeasts offer a promising route to low-alcohol and non-alcoholic beers by limiting sugar consumption and ethanol production. While some strains show potential, Saccharomyces yeasts were preferred in sensory evaluations.
Area of Science:
- Microbiology
- Fermentation Science
- Food Science
Background:
- Non-Saccharomyces yeasts are explored as alternatives for low- and non-alcoholic beer production.
- Conventional brewing often relies on Saccharomyces cerevisiae, but alternatives are sought for specific beer styles.
Purpose of the Study:
- To evaluate the potential of seven non-Saccharomyces yeasts for producing low- or non-alcoholic beers.
- To screen and select yeast strains with reduced ethanol production capabilities.
- To assess the volatile compounds and sensory profiles of the resulting beers.
Main Methods:
- Screening of non-Saccharomyces and selected Saccharomyces yeasts for sugar consumption and ethanol production.
- Selection of strains with inefficient maltose metabolism for low ethanol yield.
- Large-scale fermentations followed by volatile compound analysis and sensory evaluation.
Main Results:
- Selected yeasts produced beers with ethanol content below 1.2%.
- Detected fusel alcohols and esters in the low-alcohol beers, indicating potential for flavor development.
- Beers produced with Saccharomyces yeast received higher acceptance ratings from sensory panelists.
Conclusions:
- Non-conventional yeasts are suitable for developing low- and non-alcoholic beers.
- Further research into non-conventional yeasts can lead to novel beer varieties.
- Saccharomyces yeasts may still be preferred for certain sensory attributes in low-alcohol lagers.
More Related Videos
Related Concept Videos
Microbial Fermentation
121
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
121
Fermentation
116.7K
Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
116.7K
Fates of Pyruvate
8.8K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.8K
Yeast Signaling
14.7K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
14.7K

