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Related Experiment Videos

Studies on the intestinal surface acid microclimate: developmental aspects.

H M Said1, R Smith, R Redha

  • 1Department of Pediatric Gastroenterology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.

Pediatric Research
|November 1, 1987
PubMed
Summary
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The intestinal surface acid microclimate (ISAM) exists in developing rats. Its pH is influenced by factors like mucus, sodium, and metabolism, indicating its importance in the young gut.

Area of Science:

  • Gastroenterology
  • Developmental Biology
  • Physiology

Background:

  • The intestinal surface acid microclimate (ISAM) is a crucial physiological feature.
  • Understanding ISAM in the developing intestine is important for pediatric health.
  • Previous studies established ISAM in adult rats, but its characteristics in developing animals were less understood.

Purpose of the Study:

  • To investigate the existence and characteristics of the ISAM in the developing intestine of suckling and weanling rats.
  • To determine the factors influencing ISAM pH in the developing rat jejunum.

Main Methods:

  • In vitro pH measurements using a sensitive glass pH-microelectrode.
  • Comparison of ISAM pH across different intestinal segments (jejunum, ileum, colon) and age groups (suckling, weanling, adult).

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  • Assessment of the impact of various substances and conditions (N-acetyl-L-cysteine, stirring, Na+ removal, glucose removal, metabolic inhibitors) on jejunal ISAM pH.

    • Main Results:

    • The ISAM was confirmed in both suckling and weanling rat intestines.
    • ISAM pH was lower in the jejunum than the ileum in both age groups.
    • Colon ISAM pH was significantly lower in suckling rats compared to weanling and adult rats.
    • Jejunal ISAM pH in weanling rats was not in equilibrium with the buffer pH and was significantly affected by mucolytic agents, stirring, Na+ and glucose removal, and metabolic inhibitors.

    Conclusions:

    • The ISAM is present and functional in the developing rat intestine.
    • The ISAM is dependent on Na+, metabolizable substrates, and normal intracellular metabolism.
    • Intestinal surface mucus plays a role in maintaining the ISAM, likely by retaining hydrogen ions.