Related Experiment Video
Updated: Aug 8, 2025

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth
Published on: June 28, 2019
Cell-sized asymmetric phospholipid-amphiphilic protein vesicles with growth, fission, and molecule transportation
1Division of Molecular Science, Graduate School of Science and Technology, Gunma University, 1-5-1 Tenjin-Cho, Kiryu, Gunma 376-8515, Japan.
Abstract:
Lipid vesicles, which mimic cell membranes in structure and components, have been used to study the origin of life and artificial cell construction. A different approach to developing cell-mimicking systems focuses on the formation of protein- or polypeptide-based vesicles. However, micro-sized protein vesicles that are similar in membrane dynamics to the cell and that reconstitute membrane proteins are difficult to form. In this study, we generated cell-sized asymmetric phospholipid-amphiphilic protein (oleosin) vesicles that allow the reconstitution of membrane proteins and the growth and fission of vesicles. These vesicles are composed of a lipid membrane on the outer leaflet and an oleosin membrane on the inner leaflet. Further, we elucidated a mechanism for the growth and fission of cell-sized asymmetric phospholipid-oleosin vesicles by feeding phospholipid micelles. Our asymmetric phospholipid-oleosin vesicles with the advantages of the lipid leaflet and the protein leaflet will potentially promote understanding of biochemistry and synthetic biology.
Related Concept Videos
Clathrin Coated Vesicles
Pinching-off of Coated Vesicles
COP Coated Vesicles
Intralumenal Vesicles and Multivesicular Bodies
Vesicular Tubular Clusters
With the help of motor proteins such...
Overview of Secretory Vesicles
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...

