Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Aug 8, 2025

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing
05:34

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

Published on: February 10, 2023

1.8K

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing.

Peibo Wang1, Yongcan Zhou2, Bei Wang3

  • 1State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University.

Journal of Visualized Experiments : Jove
|February 27, 2023
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beneficial effects of Alterion supplementation on growth metrics, intestinal histomorphology, and microbial communities in indigenous and commercial chicken breeds.

Frontiers in veterinary science·2025
Same author

Liver and intestine transcriptome analysis reveals molecular mechanisms of phytase-driven nutrient utilization and metabolic regulation in hybrid catfish.

Comparative biochemistry and physiology. Part D, Genomics & proteomics·2025
Same author

Sterilization of Channel Catfish (Ictalurus punctatus) via Overexpression of bax Gene Regulated by a Tet-off System in the Primordial Germ Cells.

Marine biotechnology (New York, N.Y.)·2025
Same author

Milk yield prediction in Friesian cows using linear and flexible discriminant analysis under assumptions violations.

BMC veterinary research·2024
Same author

Reversible Sterilization of Channel Catfish via Overexpression of Glutamic Acid Decarboxylase Gene.

Animals : an open access journal from MDPI·2024
Same author

Intestinal microbiota and gene expression alterations in leopard coral grouper (Plectropomus leopardus) under enteritis.

Fish & shellfish immunology·2024

Researchers optimized a protocol for preparing high-quality single-cell suspensions from Nile tilapia intestine. This method ensures sufficient viable cells for single-cell sequencing studies in aquaculture research.

Area of Science:

  • Aquaculture
  • Cell Biology
  • Genomics

Background:

  • Nile tilapia is a key freshwater fish model in aquaculture research.
  • High-quality single-cell suspensions are crucial for advanced analyses like single-cell RNA sequencing.
  • Existing protocols are insufficient for tilapia intestine tissue.

Purpose of the Study:

  • To develop and optimize a reliable protocol for generating single-cell suspensions from Nile tilapia intestine.
  • To ensure cell viability and concentration suitable for single-cell sequencing applications.

Main Methods:

  • Optimized tissue dissociation using a collagenase/dispase enzyme combination.
  • Incorporated bovine serum albumin and DNase to minimize cell aggregation.
  • Evaluated cell viability and concentration.

More Related Videos

Author Spotlight: Isolating and Analyzing Intestinal Cells of Zebrafish Larvae for Investigating Transcriptomic Aspects of Gastrointestinal Development
06:36

Author Spotlight: Isolating and Analyzing Intestinal Cells of Zebrafish Larvae for Investigating Transcriptomic Aspects of Gastrointestinal Development

Published on: November 10, 2023

1.9K
Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights
08:23

Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights

Published on: June 28, 2024

871

Related Experiment Videos

Last Updated: Aug 8, 2025

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing
05:34

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

Published on: February 10, 2023

1.8K
Author Spotlight: Isolating and Analyzing Intestinal Cells of Zebrafish Larvae for Investigating Transcriptomic Aspects of Gastrointestinal Development
06:36

Author Spotlight: Isolating and Analyzing Intestinal Cells of Zebrafish Larvae for Investigating Transcriptomic Aspects of Gastrointestinal Development

Published on: November 10, 2023

1.9K
Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights
08:23

Author Spotlight: Integrating Single-Cell Transcriptomics with Organoid Cultures for Advanced Research and Therapeutic Insights

Published on: June 28, 2024

871

Main Results:

  • Achieved a high-quality single-cell suspension from Nile tilapia intestine.
  • Demonstrated 90% cell viability and high cell concentration, meeting single-cell sequencing requirements.
  • The protocol is adaptable for other fish species' intestines.

Conclusions:

  • The optimized protocol provides an efficient method for tilapia intestine single-cell preparation.
  • This advancement supports single-cell level studies in aquaculture fish research.
  • The protocol serves as a valuable reference, reducing experimental trial-and-error.