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Updated: Aug 8, 2025

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle
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Published on: February 1, 2017

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PPIases Par14/Par17 Affect HBV Replication in Multiple Ways

Kyongmin Kim1,2

  • 1Department of Microbiology, Ajou University School of Medicine, Suwon 16499, Republic of Korea.

Viruses
|February 28, 2023
PubMed

Insights

Human parvulin 14 (Par14) and parvulin 17 (Par17) are enzymes that boost hepatitis B virus (HBV) replication. They interact with viral proteins HBc and HBx, enhancing viral stability and assembly.

Area of Science:

  • Hepatology
  • Virology
  • Molecular Biology

Background:

  • Human parvulin 14 (Par14) and parvulin 17 (Par17) are peptidyl-prolyl cis/trans isomerases.
  • These enzymes play a role in regulating hepatitis B virus (HBV) replication.
  • Their interaction is dependent on the presence of the hepatitis B virus protein X (HBx).

Purpose of the Study:

  • To elucidate the mechanism by which Par14 and Par17 upregulate HBV replication.
  • To investigate the specific interactions between Par14/Par17, HBc, HBx, and cccDNA.
  • To identify the key residues involved in these interactions and their functional consequences.

Main Methods:

  • Analysis of protein-protein interactions between Par14/Par17, HBc, and HBx.
  • Investigation of the role of specific amino acid residues in Par14/Par17, HBc, and HBx.
  • Assessment of the impact on HBV replication, viral protein stability, and cccDNA transcription.

Main Results:

  • Par14/Par17 bind to conserved Arg-Pro motifs in HBc and HBx, enhancing viral protein stability and core particle assembly.
  • Par14/Par17 interaction with HBx promotes its translocation to the nucleus and mitochondria.
  • In the presence of HBx, Par14/Par17 are recruited to cccDNA, promoting transcriptional activation and HBc recruitment via specific residues.

Conclusions:

  • Par14 and Par17 significantly upregulate HBV replication through complex interactions involving HBx, HBc, and cccDNA.
  • These interactions occur in both the nucleus (cccDNA complex) and cytoplasm (core particles).
  • Specific binding residues on Par14/Par17 are crucial for mediating these pro-viral effects.
Keywords:
HBV replicationHBcHBxPIN4cccDNAcore particlehepatitis B virusparvulin 14parvulin 17peptidyl-prolyl cis/trans isomerase

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