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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

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Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Rapid Determination of Antibody-Antigen Affinity by Mass Photometry
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Protocol to determine antibody affinity and concentration in complex solutions using microfluidic antibody affinity

Marc Emmenegger1, Roland Worth2, Sebastian Fiedler2

  • 1Institute of Neuropathology, University of Zurich, 8091 Zurich, Switzerland.

STAR Protocols
|February 28, 2023
PubMed
Summary

This study introduces a microfluidic method for antibody affinity profiling in complex human samples. This technique measures both antibody affinity and target protein concentration, offering a powerful tool for various biomedical applications.

Keywords:
AntibodyHealth SciencesImmunologyProtein BiochemistryProteomicsSurface Plasmon Resonance (SPR)

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Area of Science:

  • Biochemistry
  • Microfluidics
  • Immunology

Background:

  • Conventional affinity measurement methods face limitations including artificial immobilization, large sample volumes, and homogeneous solutions.
  • Accurate assessment of antibody-antigen interactions is crucial for understanding immune responses and developing diagnostics or therapeutics.

Purpose of the Study:

  • To present a novel microfluidic protocol for antibody affinity profiling in complex human samples.
  • To enable simultaneous measurement of both antibody affinity and active target protein concentration.
  • To demonstrate the protocol's application in analyzing antibody responses against SARS-CoV-2 variants.

Main Methods:

  • Development and application of a microfluidic-based assay for antibody affinity profiling.
  • Utilizing complex human samples in solution, avoiding artificial immobilization.
  • Analysis of antibody-antigen interactions and active protein concentration through a unique fingerprinting approach.

Main Results:

  • The microfluidic protocol successfully profiles antibody affinity and active target protein concentration in complex human samples.
  • Demonstrated analysis of antibody responses in SARS-CoV-2 omicron-naïve samples against different variants of concern.
  • The developed fingerprint reflects both affinity and active concentration, providing comprehensive interaction data.

Conclusions:

  • The microfluidic antibody affinity profiling protocol overcomes limitations of conventional methods.
  • This technology offers a versatile platform for a broad spectrum of biomedical research questions.
  • The method provides a detailed fingerprint of antibody-antigen interactions in native sample matrices.