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Updated: Aug 8, 2025
Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay
Published on: January 31, 2022
Optimized protocol for quantification of mitochondrial non-heme and heme iron content in mouse tissues and cultured
Tatsuya Sato1, Hsiang-Chun Chang2, Konrad T Sawicki2
1Feinberg Cardiovascular Research Institute, Northwestern University School of Medicine, Chicago, IL, USA; Department of Cellular Physiology and Signal Transduction, Sapporo Medical University School of Medicine, Sapporo, Japan.
Abstract:
Impaired mitochondrial iron metabolism is associated with aging and a variety of diseases, and there is a growing need to accurately quantify mitochondrial iron levels. This protocol provides an optimized method for evaluating non-heme and heme iron in mitochondrial and cytosolic fractions of tissues and cultured cells. Our protocol consists of three steps: sample fractionation, non-heme iron measurement, and heme iron measurement. For complete details on the use and execution of this protocol, please refer to Sato et al. (2022).1.
