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Updated: Aug 8, 2025
Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle
Published on: October 26, 2017
Protocol for quantitative evaluation of the impact of paracrine senescence on cellular reprogramming in cultured
Jérémy Chantrel1, Cheng Chen2, Jun Zhang3
1Cellular Plasticity in Age-related Pathologies, Department of Developmental & Stem Cell Biology, CNRS UMR 3738, Université Paris Cité, Institut Pasteur, 25 rue du Dr Roux, 75015 Paris, France; Sorbonne Université, Collège Doctoral, 75005 Paris, France.
Abstract:
We present a protocol to evaluate the impact of senescence secretome on reprogramming to pluripotency using both cellular and mouse models. First, we describe the in vitro reprogramming procedure using conditioned medium derived from senescent cells. Next, to explore the impact of senescence on in vivo reprogramming, we detail the steps to identify senescent and reprogrammed cells in mouse skeletal muscle, followed by semi-automatic quantification. This protocol can be used to study the effect of paracrine senescence on cellular plasticity. For complete details on the use and execution of this protocol, please refer to von Joest et al. (2022).1.
