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Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
Published on: October 6, 2019
CPR5 positively regulates pattern-triggered immunity via a mediator protein
Miaomiao Ma1,2,3, Meng Li1, Rongfang Zhou1
1State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.
Constitutive Expression of PR Genes 5 (CPR5) protein is crucial for plant pattern-triggered immunity (PTI) in Arabidopsis. CPR5 negatively regulates effector-triggered immunity (ETI), with MED4 identified as a key mediator in CPR5
Area of Science:
- Plant immunity
- Molecular plant-pathogen interactions
- Cellular signaling
Background:
- Plant immune system comprises pattern-triggered immunity (PTI) and effector-triggered immunity (ETI).
- CONSTITUTIVE EXPRESSION OF PR GENES 5 (CPR5) protein negatively regulates ETI.
- The role of CPR5 in PTI remains largely uncharacterized.
Purpose of the Study:
- To investigate the role of CPR5 in plant pattern-triggered immunity (PTI).
- To identify genetic components involved in CPR5-mediated regulation of PTI.
Main Methods:
- Arabidopsis thaliana forward-genetic screen for suppressors of cpr5.
- Analysis of Pseudomonas syringae pv. tomato DC3000 hrcC- resistance assays.
- Genetic analysis of CPR5 and MED4 interactions.
Main Results:
- CPR5 is essential for the activation of various PTI responses in Arabidopsis.
- MED4 was identified as a suppressor of cpr5, restoring defective PTI.
- CPR5 plays opposing roles in regulating PTI and ETI.
Conclusions:
- CPR5 is a positive regulator of PTI, contrasting its negative role in ETI.
- MED4 is a novel genetic regulator of PTI, acting downstream or in parallel to CPR5.
- CPR5 genetically controls PTI through MED4 in Arabidopsis.
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