An "up-promotor" mutation affecting the acetamidase of Aspergillus nidulans
Abstract:
A derivative of an areA200 strain of Aspergillus nidulans selected for strong growth on acetamide as the sole nitrogen source was found to have a mutation, amd-18, closely linked to amdS, the acetamidase structural gene. This mutation results in 2--3 fold higher acetamidase activities than wildtype strains in uninduced as well as induced cultures. The effects of the amd-18 mutation are superimposed on the effects of other regulatory mutations affecting the acetamidase. The amd-18 mutation is cis-dominant with respect to the amdS gene. Immunological and gel electrophoresis studies have shown that amd-18 strains produce higher levels of acetamidase protein. A previously described cis-dominant mutation, amdI9, also causes elevated levels of acetamidase protein. The amd-18 mutation may affect a promoter site for the amdS gene allowing an increased frequency of transcription.
More Related Videos
07:32Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
Published on: March 19, 2015
09:21Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.
Published on: February 15, 2019
Related Concept Videos
Mutations
Nonsense-mediated mRNA Decay
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
The Eukaryotic Promoter Region
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Allosteric Proteins-ATCase
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis pathway,...
Fungal Phylum Ascomycota
