Related Experiment Video

Updated: Aug 8, 2025

Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis
08:55

Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis

Published on: August 7, 2018

10.9K

Mixed lineage kinase domain-like pseudokinase: Conventional (necroptosis) and unconventional

Tuna Onal1, Melike Ozgul-Onal2, Ilana Chefetz3

  • 1Faculty of Medicine, Department of Histology and Embryology, Bandirma Onyedi Eylul University, Balikesir, Turkey; The Hormel Institute, University of Minnesota, Austin, MN, United States.

Advances in Protein Chemistry and Structural Biology
|March 1, 2023
PubMed

Insights

Mixed lineage kinase domain-like pseudokinase (MLKL) is crucial for programmed cell necrosis (necroptosis). Understanding MLKL

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Pathology

Background:

  • Mixed lineage kinase domain-like pseudokinase (MLKL) is the key mediator of necroptosis, a programmed cell death pathway.
  • Necroptosis is a caspase-independent cell death mechanism implicated in inflammatory diseases and cancer.
  • Targeting necroptosis offers a potential strategy against apoptosis-resistant cancers.

Purpose of the Study:

  • To review the diverse intracellular functions of MLKL.
  • To explore MLKL's roles in both necroptosis-dependent and independent cellular processes.
  • To highlight MLKL's involvement in autophagy, inflammation, tissue regeneration, and endosomal trafficking.

Main Methods:

  • Literature review of studies on MLKL function.
  • Analysis of necroptosis signaling pathways.
  • Examination of MLKL's role in various cellular processes.

Main Results:

  • MLKL is essential for necroptosis execution.
  • MLKL regulates cellular processes beyond necroptosis.
  • MLKL is implicated in autophagy, inflammation, tissue repair, and endosomal dynamics.

Conclusions:

  • MLKL possesses multifaceted roles in cellular functions.
  • Further research into MLKL's functions can lead to novel therapeutic targets.
  • Understanding MLKL is vital for developing new treatments for diseases involving necroptosis and inflammation.
Keywords:
AutophagyEndosomal traffickingInflammationMLKLNecroptosis

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