Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Aug 8, 2025

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

Initial recommendations for performing, benchmarking and reporting single-cell proteomics experiments.

Laurent Gatto¹, Ruedi Aebersold², Juergen Cox³

¹Computational Biology and Bioinformatics Unit, de Duve Institute, Université Catholique de Louvain, Brussels, Belgium.

|March 2, 2023

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Translating Innovation to Clinic: End-to-End Bioprocess Development and cGMP Manufacturing of N332-GT5 HIV Vaccine Candidate for First-in-Human Trials HVTN144.

bioRxiv : the preprint server for biology·2026

Same author

Alternate RNA decoding results in stable and abundant proteins in mammals.

Nature·2026

Same author

Extracellular Vesicles from Glioblastoma Cells Reflect 2D vs. 3D Culture Adaptation and Resistance to Temozolomide.

Molecular & cellular proteomics : MCP·2026

Same author

Mass Spectrometry Imaging in ACS Journals.

ACS measurement science au·2026

Same author

Architecture and function of holocentric CENP-A-independent inner kinetochores.

Science advances·2026

Same author

Rapid Histone Post-Translational Modification Analysis Using Alternative Proteases and Tandem Mass Tags.

Analytical chemistry·2026

Same journal

Spatio-DARLIN enables robust and efficient in situ lineage tracing in mice at single-cell resolution.

Nature methods·2026

Same journal

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026

Same journal

Cloud-based microscope enables live neuroimaging for 24 h and beyond with worldwide access.

Nature methods·2026

Same journal

Deep molecular profiling in three dimensions.

Nature methods·2026

Same journal

3D pathology-guided microdissection.

Nature methods·2026

Same journal

Challenges and recommendations in establishing national human diversity genomic projects.

Nature methods·2026

See all related articles

Summary

This summary is machine-generated.

Analyzing single cells using tandem mass spectrometry (MS) is now possible. This study proposes best practices and quality controls to improve the accuracy and reproducibility of single-cell proteomics data.

Area of Science:

Proteomics
Cellular Biology
Analytical Chemistry

Background:

Single-cell proteomics using tandem mass spectrometry (MS) is a rapidly advancing field.
The technique offers the potential to quantify thousands of proteins across thousands of individual cells.
However, experimental design, sample preparation, data acquisition, and analysis can impact accuracy and reproducibility.

Framework:

Propose community guidelines and standardized metrics for single-cell proteomics.
Establish best practices for experimental rigor and data quality.
Recommend quality controls and data-reporting standards.

Implementation:

Develop standardized workflows for quantitative single-cell proteomics.
Facilitate alignment and data comparability between different laboratories.

More Related Videos

Author Spotlight: Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy

Author Spotlight: Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy

Published on: January 19, 2024

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

Published on: April 21, 2022

Related Experiment Videos

Last Updated: Aug 8, 2025

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Published on: December 9, 2022

Author Spotlight: Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy

Author Spotlight: Vascular Tissue Dissociation and Exploring Single-Cell Subclusters for Targeted Therapy

Published on: January 19, 2024

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

Published on: April 21, 2022

Provide resources and discussion forums for community engagement.

Implications:

Enhance the reliability and reproducibility of single-cell proteomics studies.
Accelerate the adoption of robust quantitative single-cell proteomics workflows.
Enable deeper insights into cellular heterogeneity and function.