Pickering emulsions stabilized by Chlorella pyrenoidosa protein-chitosan complex for lutein encapsulation

Lutein has many physiological functions like antioxidation, anti-cancer, and anti-inflammation, which presents good potential in the development of functional food for eye protection. However, the hydrophobicity and harsh environment factors during digestive absorption process will greatly reduce lutein bioavailability. In this study, Chlorella pyrenoidosa protein-chitosan complex stabilized Pickering emulsions were prepared, and lutein was encapsulated into corn oil droplets to increase its stability and bioavailability in gastrointestinal digestion. The interaction between Chlorella pyrenoidosa protein (CP) and chitosan (CS), and the effect of chitosan concentration on the emulsifying ability of the complex and emulsion stability were studied. With the increase of CS concentration from 0% to 0.8%, the emulsion droplet size obviously decreased, and the emulsion stability and viscosity increased significantly. In particular, when the concentration was 0.8%, the emulsion system was stable at 80 °C and 400 mM sodium chloride. After ultraviolet irradiation for 48 h, the retention rate of lutein encapsulated in Pickering emulsions was 54.33%, which was significantly higher than that (30.67%) of lutein dissolved in corn oil. The retention rate of lutein in Pickering emulsions stabilized by CP-CS complex was significantly higher than that in Pickering emulsions stabilized by CP only and corn oil after heating at 90 °C for 8 h. The results of simulated gastrointestinal digestion showed that the bioavailability of lutein encapsulated in Pickering emulsions stabilized by CP-CS complex reached 44.83%. These results explored the high-value utilization of Chlorella pyrenoidosa and provided new insights into the preparation of Pickering emulsions and the protection for lutein.