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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

3.5K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
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Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Related Experiment Video

Updated: Aug 8, 2025

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
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Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

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Combining atomic force microscopy with complementary techniques for multidimensional single-cell analysis.

Mi Li1,2,3

  • 1State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China.

Journal of Microscopy
|March 4, 2023
PubMed
Summary
This summary is machine-generated.

Atomic force microscopy (AFM) offers high-resolution imaging of biological systems. Combining AFM with other techniques provides deeper insights into single-cell analysis.

Keywords:
atomic force microscopyfluidic force microscopyinfrared spectroscopyscanning near-field ultrasound holographysingle-cell analysistip-enhanced Raman scatteringtraction force microscopy

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Area of Science:

  • Biophysics
  • Cell Biology
  • Nanotechnology

Background:

  • Atomic force microscopy (AFM) enables high-resolution characterization of biological systems in aqueous environments.
  • AFM offers unique capabilities for life science applications and integrates with complementary techniques.
  • Simultaneous sensing of multidimensional properties (biological, chemical, physical) is crucial for understanding single-cell mechanisms.

Purpose of the Study:

  • To review typical combinations of AFM with complementary techniques for single-cell analysis.
  • To highlight the applications of these integrated methods in life sciences.
  • To discuss future perspectives in the field.

Main Methods:

  • Review of literature on AFM combined with optical microscopy, ultrasound, infrared spectroscopy, Raman spectroscopy, fluidic force microscopy, and traction force microscopy.
  • Analysis of applications in single-cell studies.
  • Discussion of integrated sensing capabilities.

Main Results:

  • AFM integration with various techniques allows for comprehensive analysis of single-cell properties.
  • These combined methods offer novel possibilities for revealing mechanisms of life activities.
  • The review covers diverse applications in understanding cellular behavior.

Conclusions:

  • Integrated AFM techniques provide unprecedented spatiotemporal resolution for studying single cells.
  • Multidimensional sensing capabilities enhance the understanding of complex biological systems.
  • Future research directions for AFM-based single-cell analysis are outlined.