RhRu Alloy-Anchored MXene Nanozyme for Synergistic Osteosarcoma Therapy

Noble metal nanozymes hold promise in cancer therapy due to adjustable enzyme-like activities, unique physicochemical properties, etc. But catalytic activities of monometallic nanozyme are confined. In this study, 2D titanium carbide (Ti₃ C₂ T_x )-supported RhRu alloy nanoclusters (RhRu/Ti₃ C₂ T_x ) are prepared by a hydrothermal method and utilized for synergistic therapy of chemodynamic therapy (CDT), photodynamic therapy (PDT), and photothermal therapy (PTT) on osteosarcoma. The nanoclusters are small in size (3.6 nm), uniform in distribution, and have excellent catalase (CAT) and peroxidase (POD)-like activities. Density functional theory calculations show that there is a significant electron transfer interaction between RhRu and Ti₃ C₂ T_x , which has strong adsorption to H₂ O₂ and is beneficial to enhance the enzyme-like activity. Furthermore, RhRu/Ti₃ C₂ T_x nanozyme acts as both PTT agent for converting light into heat, and photosensitizer for catalyzing O₂ to ¹ O₂ . With the NIR-reinforced POD- and CAT-like activity, excellent photothermal and photodynamic performance, the synergistic CDT/PDT/PTT effect of RhRu/Ti₃ C₂ T_x on osteosarcoma is verified by in vitro and in vivo experiments. This study is expected to provide a new research direction for the treatment of osteosarcoma and other tumors.