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Updated: Aug 8, 2025

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
Published on: December 17, 2015
Optical coherence microscopy with a split-spectrum image reconstruction method for temporal-dynamics contrast-based
Yong Guk Kang1,2, Raymart Jay E Canoy3,2, Yongjun Jang4
1BK21 Four Institute of Precision Public Health, Korea University, Seoul 02841, Republic of Korea.
Abstract:
Biomedical researchers use optical coherence microscopy (OCM) for its high resolution in real-time label-free tomographic imaging. However, OCM lacks bioactivity-related functional contrast. We developed an OCM system that can measure changes in intracellular motility (indicating cellular process states) via pixel-wise calculations of intensity fluctuations from metabolic activity of intracellular components. To reduce image noise, the source spectrum is split into five using Gaussian windows with 50% of the full bandwidth. The technique verified that F-actin fiber inhibition by Y-27632 reduces intracellular motility. This finding could be used to search for other intracellular-motility-associated therapeutic strategies for cardiovascular diseases.
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