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Inadequate detection of the FSHR complicates future research on extragonadal FSHR localization
Victoria N Tedjawirja1, Gerrit K J Hooijer2, C Dilara Savci-Heijink2
1Department of Surgery, Amsterdam Cardiovascular Sciences, Amsterdam University Medical Centre (UMC), University of Amsterdam, Amsterdam, Netherlands.
Introduction:
Recently, follicle stimulating hormone (FSH) through interaction with its receptor (FSHR) has been proposed to play a role in postmenopausal osteoporosis and cardiovascular disease, rather than the loss of estrogen. To explore this hypothesis, unravelling which cells express extragonadal FSHR on protein level is key.
Methods:
We used two commercial anti-FSHR antibodies and validated them by performing immunohistochemistry on positive (ovary, testis) and negative controls (skin).
Results:
The monoclonal anti-FSHR antibody could not identify the FSHR in ovary or testis. The polyclonal anti-FSHR antibody stained the granulosa cells (ovary) and Sertoli cells (testis), yet there was equally intense staining of other cells/extracellular matrix. Furthermore, the polyclonal anti-FSHR antibody also stained skin tissue extensively, suggesting that the antibody stains more than just FSHR.
Discussion:
The findings in this study may add accuracy to literature on extragonadal FSHR localization and warrants attention to the use of inadequate anti-FSHR antibodies to value the potential role of FSH/FSHR in postmenopausal disease.
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