Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Leaky Scanning02:28

Leaky Scanning

5.2K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.2K
RNA Splicing01:32

RNA Splicing

56.6K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DDX3X Promotes Rotavirus Infection and Serves as an Antiviral Target.

Transboundary and emerging diseases·2025
Same author

A MERS-CoV-like mink coronavirus uses ACE2 as an entry receptor.

Nature·2025
Same author

Transcriptomic Analysis of PDCoV-Infected HIEC-6 Cells and Enrichment Pathways PI3K-Akt and P38 MAPK.

Viruses·2024
Same author

Interaction of Nipah Virus F and G with the Cellular Protein Cortactin Discovered by a Proximity Interactome Assay.

International journal of molecular sciences·2024
Same author

IFITM3 inhibits severe fever with thrombocytopenia syndrome virus entry and interacts with viral Gc protein.

Journal of medical virology·2024
Same author

CRL4B E3 ligase recruited by PRPF19 inhibits SARS-CoV-2 infection by targeting ORF6 for ubiquitin-dependent degradation.

mBio·2024

Related Experiment Video

Updated: Aug 8, 2025

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
10:00

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

Published on: March 24, 2015

13.5K

Identification of a Novel Interferon Lambda Splice Variant in Chickens.

Jing Chen1,2, Peiheng Li2, Wancheng Zou2

  • 1College of Veterinary Medicine, Key Lab for Zoonoses Research, Ministry of Education, Jilin University, Changchun, China.

Journal of Virology
|March 6, 2023
PubMed
Summary

Researchers discovered a new chicken interferon lambda, chIFNL3a, which is a splice variant that inhibits viral replication. This finding expands our understanding of avian immunity and offers potential new therapeutic directions for poultry viruses.

Keywords:
NDVNewcastle disease virusantiviralchIFNLidentificationinfluenza virus

More Related Videos

An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis
07:26

An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis

Published on: October 4, 2018

9.1K
Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
13:41

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

12.5K

Related Experiment Videos

Last Updated: Aug 8, 2025

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
10:00

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

Published on: March 24, 2015

13.5K
An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis
07:26

An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis

Published on: October 4, 2018

9.1K
Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
13:41

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

12.5K

Area of Science:

  • Immunology
  • Virology
  • Genomics

Background:

  • Type III interferons (IFNLs) are crucial for innate immunity, particularly at mucosal surfaces.
  • Limited data exists on the IFNL repertoire in avian species, with only one member previously identified in chickens (chIFNL3).
  • Interferons (IFNs) are vital immune factors classified into types I, II, and III, each using distinct receptor complexes.

Purpose of the Study:

  • To identify and characterize novel IFNLs in avian species.
  • To investigate the antiviral properties of the newly identified chicken IFNL, chIFNL3a.
  • To elucidate the role of chIFNL3a in the chicken immune response against viral infections.

Main Methods:

  • Identification of a novel chicken IFNL (chIFNL3a) from genomic sequences.
  • Genetic, evolutionary, and sequence analyses to determine its classification and relationship to other IFNs.
  • Activation of IFN-regulated genes and inhibition of Newcastle disease virus (NDV) and influenza virus replication in vitro, utilizing the baculovirus expression system (BES).

Main Results:

  • A novel chicken IFNL, termed chIFNL3a, was identified as a splice variant with 57.1% amino acid identity to chIFNL.
  • Phylogenetic analysis confirmed chIFNL3a clusters within the type III IFNs group.
  • chIFNL3a demonstrated the ability to activate IFN-regulated genes and significantly inhibit the replication of NDV and influenza virus in vitro.

Conclusions:

  • The discovery of chIFNL3a expands the known repertoire of chicken interferons, specifically identifying a new type III IFNL splice variant.
  • chIFNL3a possesses significant antiviral activity against important poultry pathogens like NDV and influenza virus.
  • These findings provide valuable insights into avian antiviral immunity and suggest potential avenues for developing new therapeutic strategies against viral infections in poultry.