Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Comparative Bioinformatic Analysis of Optic Nerve Axon Regeneration Lipidomes Using the <i>Xenopus laevis</i> as a Model System.

Methods and protocols·2025

Same author

Mass spectrometry dataset of LC-MS lipidomics analysis of <i>Xenopus laevis</i> optic nerve.

Data in brief·2023

Same author

Surgical Methods in Postmetamorphic Xenopus laevis: Optic Nerve Crush Injury Model.

Methods in molecular biology (Clifton, N.J.)·2023

Same author

Resting metabolic rate, abdominal fat pad and liver metabolic gene expression in female rats provided a snacking diet from weaning to adulthood.

Physiology & behavior·2022

Same author

High-fat high-sugar diet induces polycystic ovary syndrome in a rodent model.

Biology of reproduction·2017

Same author

Organophosphate pesticides induce morphological abnormalities and decrease locomotor activity and heart rate in Danio rerio and Xenopus laevis.

Environmental toxicology and chemistry·2014

Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Aug 7, 2025

Xenopus laevis as a Model to Identify Translation Impairment

Xenopus laevis as a Model to Identify Translation Impairment

Published on: September 27, 2015

Translating Ribosome Affinity Purification (TRAP) and Bioinformatic RNA-Seq Analysis in Post-metamorphic Xenopus

Gregg B Whitworth¹, Fiona L Watson²

¹Department of Biology, Washington and Lee University, Lexington, VA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|March 7, 2023

Summary

This summary is machine-generated.

This study details an updated Translating Ribosome Affinity Purification (TRAP) protocol for Xenopus laevis. The method enables isolation of specific cell type mRNAs for gene expression analysis and understanding gene networks.

Keywords:

Expression profiling RNA-Seq analysis TRAP Xenopus laevis

More Related Videos

TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

Published on: September 10, 2015

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Published on: June 27, 2013

Related Experiment Videos

Last Updated: Aug 7, 2025

Xenopus laevis as a Model to Identify Translation Impairment

Xenopus laevis as a Model to Identify Translation Impairment

Published on: September 27, 2015

TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

Published on: September 10, 2015

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Published on: June 27, 2013

Area of Science:

Developmental Biology
Molecular Biology
Genomics

Background:

Technical advances allow mRNA isolation from distinct cell types for gene expression analysis.
Comparing gene expression across different conditions reveals insights into gene networks.
Translating Ribosome Affinity Purification (TRAP) isolates ribosome-bound mRNAs from specific cell populations.

Purpose of the Study:

To provide an updated, stepwise protocol for TRAP in Xenopus laevis.
To detail experimental design, controls, and bioinformatic analysis for Xenopus translatome studies using TRAP and RNA-Seq.

Main Methods:

Updated Translating Ribosome Affinity Purification (TRAP) protocol.
Utilizing transgenic Xenopus laevis expressing a ribosomal affinity tag (ribotag).
RNA-sequencing (RNA-Seq) for translatome analysis.

Main Results:

Successfully adapted and updated the TRAP method for Xenopus laevis.
Established a protocol for isolating cell-type-specific mRNAs.
Provided a framework for bioinformatic analysis of the Xenopus translatome.

Conclusions:

The updated TRAP protocol facilitates detailed gene expression studies in Xenopus laevis.
This method aids in understanding gene networks and responses to various conditions.
TRAP combined with RNA-Seq is a powerful tool for Xenopus research.