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Related Experiment Videos

High-energy phosphates and function in isolated, working rabbit hearts.

E D Lewandowski1, M D Devous, R L Nunnally

  • 1Nuclear Medicine Center, University of Texas Health Science Center at Dallas 75235.

The American Journal of Physiology
|November 11, 1987
PubMed
Summary

This study developed a rabbit heart model for nuclear magnetic resonance (NMR) spectroscopy, showing stable function and hemodynamic responses. The model revealed rapid phosphocreatine loss and gradual ATP depletion during ischemia, with impaired functional recovery post-reperfusion.

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Area of Science:

  • Cardiovascular Physiology
  • Biophysics
  • Nuclear Magnetic Resonance Spectroscopy

Background:

  • Developing reliable experimental models is crucial for understanding cardiac metabolism and function.
  • Nuclear Magnetic Resonance (NMR) spectroscopy offers non-invasive insights into cellular energetics.
  • Previous models have limitations in stability or metabolic monitoring.

Purpose of the Study:

  • To develop and validate an isolated, working rabbit heart model for NMR spectroscopy.
  • To investigate cardiac metabolic changes, specifically ATP and phosphocreatine (PCr) levels, during global ischemia and reperfusion.
  • To correlate metabolic changes with functional recovery in the rabbit heart model.

Main Methods:

  • An isolated, working rabbit heart preparation was established and maintained for up to 4 hours.

Related Experiment Videos

  • Simultaneous measurements of hemodynamic parameters (left ventricular pressure, dP/dt, heart rate, cardiac output) and 31P NMR spectra were performed.
  • Hearts were subjected to global ischemia (13.5 min) followed by reperfusion (60 min) or chronic ischemia (45 min).

    • Main Results:

    • The heart model demonstrated functional stability and typical hemodynamic responses to workload changes.
    • During ischemia, phosphocreatine (PCr) levels rapidly decreased to undetectable levels within 11 minutes.
    • Adenosine triphosphate (ATP) levels decreased gradually and remained depressed during reperfusion, with no clear correlation to functional recovery.

    Conclusions:

    • The isolated rabbit heart model is suitable for NMR studies of cardiac metabolism and function.
    • Ischemia causes rapid PCr depletion and sustained ATP reduction, impacting functional recovery.
    • Functional recovery after ischemia is incomplete, irrespective of post-ischemic ATP levels.