Iron oxides act as an alternative electron acceptor for aerobic methanotrophs in anoxic lake sediments

Conventional aerobic CH₄-oxidizing bacteria (MOB) are frequently detected in anoxic environments, but their survival strategy and ecological contribution are still enigmatic. Here we explore the role of MOB in enrichment cultures under O₂ gradients and an iron-rich lake sediment in situ by combining microbiological and geochemical techniques. We found that enriched MOB consortium used ferric oxides as alternative electron acceptors for oxidizing CH₄ with the help of riboflavin when O₂ was unavailable. Within the MOB consortium, MOB transformed CH₄ to low molecular weight organic matter such as acetate for consortium bacteria as a carbon source, while the latter secrete riboflavin to facilitate extracellular electron transfer (EET). Iron reduction coupled to CH₄ oxidation mediated by the MOB consortium was also demonstrated in situ, reducing 40.3% of the CH₄ emission in the studied lake sediment. Our study indicates how MOBs survive under anoxia and expands the knowledge of this previously overlooked CH₄ sink in iron-rich sediments.