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LncRNA SNHG12 Decreases Non-Small Cell Lung Cancer Cell Sensitivity to Cisplatin by Repressing miR-525-5p and

Deli Tan1, Song Wang1, Peng Zhang1

  • 1Department of Thoracic Surgery, Southwest Hospital, Chongqing Ninth People's Hospital, Chongqing, China.

Annals of Clinical and Laboratory Science
|March 8, 2023
PubMed
Abstract

Insights

Long noncoding RNA SNHG12 promotes cisplatin resistance in non-small cell lung cancer by upregulating XIAP via inhibiting miR-525-5p. This finding offers potential therapeutic targets for overcoming drug resistance in NSCLC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Non-small cell lung cancer (NSCLC) is a leading cause of cancer mortality worldwide.
  • Long noncoding RNAs (lncRNAs) play a role in NSCLC progression and drug resistance.
  • Understanding the mechanisms of cisplatin resistance is crucial for improving NSCLC treatment outcomes.

Purpose of the Study:

  • To investigate the role of lncRNA SNHG12 in cisplatin (DDP) resistance in NSCLC cells.
  • To elucidate the underlying molecular mechanism involving miR-525-5p and XIAP.
  • To explore potential therapeutic strategies targeting the SNHG12/miR-525-5p/XIAP axis.

Main Methods:

  • Quantitative reverse-transcription polymerase chain reaction (RT-qPCR) to measure gene expression.
  • Cell counting kit-8 (CCK-8), colony formation, and flow cytometry assays to assess cell viability, proliferation, and apoptosis.
  • Transfection of small interfering RNAs (siRNAs), microRNA inhibitors, and plasmids.
  • Dual-luciferase reporter gene assays to confirm binding interactions.
  • Nuclear/cytosol fractionation to determine subcellular localization.

Main Results:

  • SNHG12 and XIAP were upregulated, while miR-525-5p was downregulated in NSCLC cells.
  • SNHG12 knockdown enhanced DDP sensitivity by increasing apoptosis and decreasing proliferation.
  • SNHG12 directly repressed miR-525-5p, which in turn targeted and inhibited XIAP.
  • Overexpression of XIAP or inhibition of miR-525-5p conferred DDP resistance.

Conclusions:

  • SNHG12 promotes DDP resistance in NSCLC by upregulating XIAP through the repression of miR-525-5p.
  • The SNHG12/miR-525-5p/XIAP axis is a key regulator of DDP resistance in NSCLC.
  • Targeting this axis may represent a promising therapeutic strategy for overcoming DDP resistance in NSCLC.
Keywords:
Cisplatin resistanceDual-luciferase reporter gene assayIC50LncRNA SNHG12Non-small cell lung cancerXIAPceRNAmiR-525-5p

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