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Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
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Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies
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Biomarker discovery for tuberculosis using metabolomics.

Yi Yu1, Xin-Xin Jiang2, Ji-Cheng Li2,3

  • 1Center for Analyses and Measurements, College of Chemical Engineering, Zhejiang University of Technology, Hangzhou, China.

Frontiers in Molecular Biosciences
|March 9, 2023
PubMed
Summary
This summary is machine-generated.

Metabolomics aids in discovering tuberculosis biomarkers for diagnosing active disease, latent infection, and drug resistance. Further validation is crucial for clinical application of these vital tuberculosis diagnostic tools.

Keywords:
Mycobacterium tuberculosisbiomarkerdrug resistant tuberculosismetabolomicspulmonary tuberculosistuberculosis

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Area of Science:

  • * Biochemistry and Molecular Biology
  • * Infectious Diseases and Microbiology
  • * Biomarker Discovery and Diagnostics

Background:

  • * Tuberculosis (TB) remains a leading infectious cause of death globally.
  • * Increasing drug-resistant *Mycobacterium tuberculosis* (Mtb) and progression from latent to active TB necessitate improved diagnostic and monitoring tools.
  • * Metabolomics offers quantitative metabolite profiling for host and pathogen analysis.

Purpose of the Study:

  • * To review the application of metabolomics in discovering biomarkers for tuberculosis.
  • * To highlight host-based biomarkers for active TB diagnosis, LTBI identification, risk prediction, and treatment monitoring.
  • * To discuss pathogen-based biomarkers for identifying drug-resistant TB.

Main Methods:

  • * Review of recent advancements in metabolomics applied to tuberculosis research.
  • * Analysis of studies focusing on host-derived biomarkers in body fluids (e.g., blood).
  • * Examination of research on pathogen-derived biomarkers for drug resistance detection.

Main Results:

  • * Metabolomics has identified potential biomarkers in body fluids for active TB, LTBI, and treatment response.
  • * Pathogen-based metabolomic studies show promise for detecting drug-resistant TB.
  • * Numerous candidate biomarkers require further validation and clinical testing.

Conclusions:

  • * Metabolomics is a powerful approach for tuberculosis biomarker discovery.
  • * Host and pathogen-based biomarkers can significantly improve TB diagnosis, monitoring, and drug resistance detection.
  • * Clinical validation and advanced bioinformatics are essential for translating metabolomic findings into practical applications.