Related Experiment Video

Updated: Aug 7, 2025

Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment
06:48

Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment

Published on: April 29, 2022

2.3K

Missorting of plasma miRNAs in aging and Alzheimer's disease

Maria Čarna1, Jan S Novotny1, Neda Dragišić1

  • 1International Clinical Research Centre, St. Anne's University Hospital, Brno, Czech Republic.

Journal of Neurochemistry
|March 9, 2023
PubMed

Insights

Aging and Alzheimer's disease (AD) involve microRNAs (miRNAs). In AD, circulating miRNAs are further downregulated and primarily found in extracellular vesicles, indicating a pathological worsening of aging processes.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Neuroscience

Background:

  • Aging is a primary risk factor for Alzheimer's disease (AD).
  • MicroRNAs (miRNAs) play a role in regulating aging.
  • The circulating miRNA network's role in AD, beyond aging, requires investigation.

Purpose of the Study:

  • To investigate the circulating miRNA network in Alzheimer's disease (AD) in the context of aging.
  • To understand how miRNA dysregulation in AD differs from normal aging.

Main Methods:

  • Analysis of plasma miRNA levels and their predicted localization.
  • Examination of miRNA motifs related to extracellular vesicle (EV) loading and secretion.
  • Comparison of circulating miRNA profiles in aging versus AD.

Main Results:

  • Plasma miRNAs are downregulated in aging and predicted to be within extracellular vesicles (EVs).
  • In AD, miRNAs show further downregulation and altered EV-loading/secretion motifs.
  • AD-associated miRNAs are exclusively predicted to reside within EVs.

Conclusions:

  • The circulating miRNA network in AD reflects an exacerbated aging process.
  • Physiological miRNA-mediated suppression of AD pathology becomes insufficient in AD.
  • EV-bound miRNAs are central to AD pathogenesis and aging-related decline.
Keywords:
Alzheimer's diseaseagingendosomesextracellular vesiclesmiRNAncRNAplasmashort sequence motifs

More Related Videos

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening08:14

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

Published on: October 26, 2017

15.7K
Harnessing the Power of MicroRNA Cargoes in Small Extracellular Vesicles Released from Fresh-Frozen Human Brain Sections07:55

Harnessing the Power of MicroRNA Cargoes in Small Extracellular Vesicles Released from Fresh-Frozen Human Brain Sections

Published on: November 8, 2024

487

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K