Interrogating the precancerous evolution of pathway dysfunction in lung squamous cell carcinoma using XTABLE.

Area of Science:

• Oncology
• Genomics
• Bioinformatics

Background:

• Lung squamous cell carcinoma (LUSC) has a poor prognosis and limited therapeutic options.
• Understanding premalignant lesions (PMLs) in LUSC is crucial for early detection and prevention.
• Identifying molecular drivers of malignant progression in LUSC is a key research challenge.

Purpose of the Study:

• To develop an open-source application, XTABLE, for analyzing transcriptomic data of LUSC PMLs.
• To facilitate research into the biology of LUSC PMLs and identify early detection biomarkers.
• To investigate the role of chromosomal instability and map LUSC pathways during disease progression.

Main Methods:

• Designed XTABLE, an application integrating extensive transcriptomic databases of LUSC PMLs.
• Enabled users to stratify samples and perform various biological interrogations (e.g., group comparisons, gene analysis).
• Conducted a comparative study on chromosomal instability scores and mapped LUSC pathways to PML developmental stages.

Main Results:

• XTABLE provides a comprehensive platform for exploring LUSC PML transcriptomes.
• Chromosomal instability scores were evaluated as potential biomarkers for PML progression.
• Key LUSC pathways were mapped to specific stages of LUSC development.

Conclusions:

• XTABLE significantly facilitates research on LUSC early detection biomarkers.
• The application enhances understanding of the molecular processes underlying LUSC precancerous stages.
• Further research using XTABLE can improve LUSC prevention and treatment strategies.