Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple method to detect complement receptors using baker's yeast: Y C rosettes.

I Rivero, H E Abaca, R Vallés

    Scandinavian Journal of Immunology
    |January 1, 1979
    PubMed
    Summary

    A new method uses yeast coated with complement (C) to identify complement-receptor-bearing cells. This technique aids in distinguishing various lymphocyte populations, crucial for immunological studies.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Vaccination against community acquired pneumonia in adult patients. A position paper by Neumoexpertos en Prevención].

    Semergen·2016
    Same author

    Fluorescent microspheres--a new approach to quantifying live feed intake in larval fish.

    Communications in agricultural and applied biological sciences·2014
    Same author

    Changes in UCP2, PPARgamma2, and c/EBPalpha gene expression induced by a neuropeptide Y (NPY) related receptor antagonist in overweight rats.

    Nutritional neuroscience·2002
    Same author

    A new NPY-antagonist strongly stimulates apoptosis and lipolysis on white adipocytes in an obesity model.

    Life sciences·2000
    Same author

    [Peripheral arteriopathy in type 2 diabetes mellitus].

    Atencion primaria·1998
    Same author

    Signet-ring squamous cell carcinoma: a case report.

    Journal of cutaneous pathology·1998

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Identifying specific cell surface receptors is crucial for understanding immune cell function.
    • Complement receptors play a significant role in immune responses and cell signaling.

    Purpose of the Study:

    • To describe a novel technique for identifying complement-receptor-bearing cells.
    • To establish a method for enumerating distinct lymphocyte populations based on receptor expression.

    Main Methods:

    • Utilized heat-killed baker's yeast treated with fresh serum as a ligand.
    • Incubation of serum-coated yeast with cells to form rosettes.
    • Investigated the role of complement factors (specifically C3) in rosette formation.

    Main Results:

    • Demonstrated that complement factors are essential for yeast-cell attachment (rosette formation).

    Related Experiment Videos

  • Established a mean rosette formation rate of 11.6% in normal lymphocytes.
  • Showcased the ability to identify four lymphocyte populations using a combined rosette technique.

    • Conclusions:

    • The described yeast-complement (YC) rosette technique effectively identifies complement-receptor-bearing lymphocytes.
    • This method provides a reliable way to enumerate lymphocyte subsets, including those with dual receptors.
    • The technique is valuable for immunological research and clinical diagnostics.