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The basic proteins of bovine allantoic fluid
P P Lambert1, M Lammens-Verslijpe, J Sennesael
1Queen Elisabeth Medical Foundation, Brussels, Belgium.
Biology of Reproduction
|November 1, 1987
Summary
Highly cationic proteins in bovine allantoic fluid were isolated and characterized. These proteins, particularly those with a high isoelectric point (pI), show potential pathogenic properties, including inducing blood coagulation and hemolysis.
Area of Science:
- Biochemistry
- Veterinary Science
Background:
- Bovine allantoic fluid contains proteins, including highly cationic ones.
- Understanding these proteins is crucial for assessing potential health impacts.
Purpose of the Study:
- To isolate and characterize highly cationic proteins from bovine allantoic fluid.
- To investigate the potential pathogenic properties of these isolated proteins.
Main Methods:
- Ion-exchange chromatography, chromatofocusing, heparin-sepharose binding, and gel filtration were used for protein separation.
- Polyacrylamide gel electrophoresis in sodium dodecyl sulfate was employed for analysis.
- Protein components were identified by molecular weight (Mr) and isoelectric point (pI).
Main Results:
- Several protein fractions were separated, with two major components (Mr 34,000, pI 9.7 and Mr 38,000, pI 9.6-9.0) comprising 33% of basic proteins.
- These major basic proteins were not found in ox plasma and bound to red cells and platelets, decreasing cell electrophoretic mobility.
- While high pI polycations induced coagulation and hemolysis, the isolated major basic proteins did not cause significant red cell clumping or hemolysis at tested concentrations.
Conclusions:
- Bovine allantoic fluid contains distinct highly cationic proteins not present in plasma.
- These proteins interact with blood cells, but their pathogenic potential in vivo requires further investigation.
- The study provides a basis for understanding the biochemical and potential biological roles of these proteins.