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Related Experiment Videos

The basic proteins of bovine allantoic fluid.

P P Lambert1, M Lammens-Verslijpe, J Sennesael

  • 1Queen Elisabeth Medical Foundation, Brussels, Belgium.

Biology of Reproduction
|November 1, 1987
PubMed
Summary

Highly cationic proteins in bovine allantoic fluid were isolated and characterized. These proteins, particularly those with a high isoelectric point (pI), show potential pathogenic properties, including inducing blood coagulation and hemolysis.

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Area of Science:

  • Biochemistry
  • Veterinary Science

Background:

  • Bovine allantoic fluid contains proteins, including highly cationic ones.
  • Understanding these proteins is crucial for assessing potential health impacts.

Purpose of the Study:

  • To isolate and characterize highly cationic proteins from bovine allantoic fluid.
  • To investigate the potential pathogenic properties of these isolated proteins.

Main Methods:

  • Ion-exchange chromatography, chromatofocusing, heparin-sepharose binding, and gel filtration were used for protein separation.
  • Polyacrylamide gel electrophoresis in sodium dodecyl sulfate was employed for analysis.
  • Protein components were identified by molecular weight (Mr) and isoelectric point (pI).

Main Results:

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  • Several protein fractions were separated, with two major components (Mr 34,000, pI 9.7 and Mr 38,000, pI 9.6-9.0) comprising 33% of basic proteins.
  • These major basic proteins were not found in ox plasma and bound to red cells and platelets, decreasing cell electrophoretic mobility.
  • While high pI polycations induced coagulation and hemolysis, the isolated major basic proteins did not cause significant red cell clumping or hemolysis at tested concentrations.

Conclusions:

  • Bovine allantoic fluid contains distinct highly cationic proteins not present in plasma.
  • These proteins interact with blood cells, but their pathogenic potential in vivo requires further investigation.
  • The study provides a basis for understanding the biochemical and potential biological roles of these proteins.