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Pre CFU-F in bone marrow cultures from children with hematopoietic disease including acute leukemia
G Prindull1, Z Ben-Ishay, B Prindull
1Department of Pediatrics, University of Göttingen, Federal Republic of Germany.
Insights
Bone marrow stromal cells in children support the growth of fibroblast precursor cells (CFU-F). These cells may act as a sanctuary for clonogenic cells in both hematopoietic and fibroblast lineages.
Area of Science:
- Hematology
- Cell Biology
- Oncology
Background:
- Bone marrow stromal cells play a critical role in hematopoiesis.
- Understanding stromal cell function is crucial for leukemia research.
Purpose of the Study:
- To investigate stromal precursor cells (CFU-F) in pediatric bone marrow.
- To characterize clonogenic fibroblast precursor cells in bone marrow cultures.
Main Methods:
- Culture of bone marrow aspirates from children.
- Colony-forming unit assays (CFU-F, CFU-GM).
- Monoclonal antibody staining for cell identification.
Main Results:
- Normal and leukemia remission samples showed similar CFU-F growth.
- Active leukemia patients exhibited reduced CFU-F growth.
- Immature fibroblast precursor cells (pre CFU-F) and round cells were identified after prolonged culture.
Conclusions:
- Bone marrow stromal cells support fibroblast precursor growth.
- A sanctuary role for the stromal matrix is proposed for clonogenic cells.
- This finding has implications for understanding leukemia microenvironments.
Abstract:
Stromal precursor cells from bone marrow aspirates of children have been studied in culture. In 7 day liquid cultures normal individuals and patients with acute leukemia in remission grew 110 +/- 50 CFU-F and 100 +/- 40 CFU-F (colony forming unit--fibroblasts) respectively, per 6 X 10(5) buffy coat mononuclear cells. Staining with monoclonal antibodies suggests that stromal cells from CFU-F colonies are fibroblasts. CFU-F colony growth from the bone marrow of patients with active leukemia was low. After cultivation periods of more than 21 days, we observed, in addition, still more immature, clonogenic fibroblast precursor cells, "pre CFU-F", and round cells attached to stromal cells from pre CFU-F colonies. From the round cells, we have passaged pre CFU-F and CFU-GM (colony forming unit--granulocytic, monocytic) in secondary cultures. Our observations are in agreement with the concept that the bone marrow stromal cell matrix serves as a sanctuary for reversibly attached clonogenic cells of both the hematopoietic and fibroblast lineages.