Related Experiment Video
Updated: Aug 7, 2025
11:56
Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species
Published on: April 17, 2009
21.1K
Dissecting the Meiotic Recombination Patterns in a Brassica napus Double Haploid Population Using 60K SNP Array
Shuxiang Yan1, Jianjie He1, Mi Tang1
1Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
International Journal of Molecular Sciences
|March 11, 2023
Summary
Researchers mapped meiotic recombination in Brassica napus using a 60K SNP array. This study identified key recombination hotspots linked to seed oil content, aiding future crop improvement strategies.
Area of Science:
- Plant genetics and genomics
- Molecular biology
- Agricultural science
Background:
- Meiotic recombination is crucial for chromosome stability and genetic variation, essential for crop adaptation and improvement.
- Understanding crossover (CO) patterns in populations like Brassica napus is vital for breeding, but cost-effective methods are limited.
- Previous studies lacked comprehensive population-level recombination data for Brassica napus.
Purpose of the Study:
- To systematically investigate the recombination landscape in a Brassica napus population.
- To identify recombination hotspots and their relationship with gene expression and phenotypic variation.
- To provide a foundation for improving crop traits like seed oil content through marker-assisted selection.
Main Methods:
- Utilized the Brassica 60K Illumina Infinium SNP array to analyze recombination frequency across the genome in a double haploid (DH) population of Brassica napus.
- Mapped crossover (CO) distribution, identified CO hotspots, and analyzed gene expression in relation to recombination frequency.
- Constructed a recombination bin map and identified specific bins associated with seed oil content variation.
Main Results:
- Crossover events were unevenly distributed, with higher frequencies at chromosome ends (distal regions).
- Over 30% of genes in CO hotspots were associated with plant defense and regulation; gene expression was higher in hotspots.
- Specific recombination bins on chromosomes A08, A09, C03, and C06 were significantly associated with seed oil content variation.
Conclusions:
- The study provides a detailed map of meiotic recombination in Brassica napus at the population level.
- Identified genomic regions and genes associated with recombination frequency and important agronomic traits like seed oil content.
- Offers valuable insights and tools for future rapeseed breeding programs and comparative studies in other species.
More Related Videos
06:31An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
Published on: January 11, 2017
9.8K
09:32An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants
Published on: November 8, 2017
7.9K
Related Concept Videos
01:05Single Nucleotide Polymorphisms-SNPs
15.4K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
15.4K
01:30Crossing Over
4.6K
Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I,...
4.6K
02:08Gene Conversion
9.8K
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
9.8K
02:53Conservative Site-specific Recombination and Phase Variation
6.1K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
6.1K
02:31Homologous Recombination
50.8K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.8K