A Bifunctional Fluorescence Probe Based on AIE-ICT Strategy for Visual Detection of Cu²⁺/Co²⁺ in Complex Matrix

A novel fluorescence chemical sensor-based probe 1-{[(E)-(2-aminophenyl)azanylidene]methyl}naphthalen-2-ol (AMN) was designed and synthesized, which performed a "naked eye" detection ability toward Cu²⁺ and Co²⁺ based on aggregation-induced emission (AIE) fluorescence strategy. It has sensitive detection ability for Cu²⁺ and Co²⁺. In addition, the color changed from yellow-green to orange under the sunlight, realizing the rapid identification of Cu²⁺/Co²⁺, which has the potential of on-site visual detection under the "naked eye". Moreover, different "on" and "off" fluorescence expressions were exhibited under excessive glutathione (GSH) in AMN-Cu²⁺ and AMN-Co²⁺ systems, which could be employed to distinguish Cu²⁺ from Co²⁺. The detection limits for Cu²⁺ and Co²⁺ were measured to be 8.29 × 10^-8 M and 9.13 × 10^-8 M, respectively. The binding mode of AMN was calculated to be 2:1 by Jobs' plot method analysis. Ultimately, the new fluorescence sensor was applied to detect Cu²⁺ and Co²⁺ in real samples (tap water, river water, and yellow croaker), and the results were satisfying. Therefore, this high-efficiency bifunctional chemical sensor platform based on "on-off" fluorescence detection will provide significant guidance for the advance development of single-molecule sensors for multi-ion detection.