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Related Concept Videos

DNA Isolation01:24

DNA Isolation

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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Updated: Aug 7, 2025

Composition and Distribution Analysis of Bioaerosols Under Different Environmental Conditions
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Composition and Distribution Analysis of Bioaerosols Under Different Environmental Conditions

Published on: January 7, 2019

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An Optimized Active Sampling Procedure for Aerobiological DNA Studies.

Jyothi Basapathi Raghavendra1, Thasshwin Mathanlal1, Maria-Paz Zorzano2

  • 1Department of Planetary Sciences, School of Geosciences, University of Aberdeen, Aberdeen AB24 3UE, UK.

Sensors (Basel, Switzerland)
|March 11, 2023
PubMed
Summary
This summary is machine-generated.

A new portable bioaerosol sampler effectively captures airborne microbial DNA for real-time genomic monitoring. This system overcomes low biomass challenges, enabling continuous environmental surveillance of microbial communities.

Keywords:
DNA extractionactive samplingair-filtrationbioaerosolscommercial off-the shelf (COTS)

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Area of Science:

  • Environmental Science
  • Microbiology
  • Genomics

Background:

  • Atmospheric microbial biomass is low, making real-time monitoring of airborne microbial communities difficult.
  • Low deoxyribose nucleic acid (DNA) and protein concentrations in the atmosphere are easily contaminated by operators and instruments.
  • Genomic studies offer sensitive and rapid methods for tracking bioaerosol composition changes.

Purpose of the Study:

  • To design and demonstrate an optimized, portable, closed bioaerosol sampler for autonomous, long-term outdoor operation.
  • To identify the optimal membrane filter and DNA extraction kit for capturing and recovering airborne microbial DNA.
  • To establish a methodology for continuous environmental monitoring of microbial communities in the air.

Main Methods:

  • Developed a portable, closed bioaerosol sampler using commercial components and membrane filters.
  • Utilized a bioaerosol chamber for comparative analysis of membrane filters and DNA extraction kits.
  • Conducted a 24-hour outdoor field test of the sampler at 150 L/min.

Main Results:

  • A 0.22-µm polyether sulfone (PES) membrane filter was identified as optimal for DNA capture and extraction.
  • The optimized sampler successfully captured ambient bioaerosols, avoiding operator contamination.
  • Up to 4 ng of DNA was recovered over 24 hours, sufficient for genomic applications.

Conclusions:

  • The developed bioaerosol sampler and extraction protocol provide a robust system for environmental monitoring.
  • Automation of this system will enable continuous insights into the temporal dynamics of airborne microbial communities.
  • This technology addresses the challenge of low biomass in atmospheric samples for genomic analysis.