In Vivo Analysis of Dendritic Cell Clonality
1Immunobiology Laboratory, The Francis Crick Institute, London, UK. mdmc2@cam.ac.uk.
Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2023
Summary
This study presents a multicolor fate mapping technique to track conventional dendritic cell (cDC) progeny in tissues. The method aids in analyzing cell distribution and understanding cDC clonality using advanced microscopy.
Area of Science:
- Immunology
- Cell Biology
- Microscopy
Background:
- Confocal fluorescence microscopy is vital for analyzing tissue architecture and cell distribution.
- Multicolor fate mapping combined with microscopy enables the study of cell lineage and clonal relationships.
Purpose of the Study:
- To describe a multicolor fate mapping mouse model and microscopy technique.
- To trace the progeny of conventional dendritic cell (cDC) progenitors in various tissues.
- To analyze cDC clonality and understand their behavior within tissues.
Main Methods:
- Development of a multicolor fate mapping mouse model.
- Application of confocal fluorescence microscopy for high-resolution imaging.
- Focus on imaging methodologies for tracing cell lineages.
Main Results:
- The described technique allows for the visualization and tracing of conventional dendritic cell (cDC) progeny.
- The method facilitates the analysis of cell clusters and informs on clonal relationships.
- Imaging protocols are detailed for studying cDC clonality.
Conclusions:
- This chapter provides a robust method for studying conventional dendritic cell (cDC) development and behavior.
- The described imaging techniques are crucial for understanding tissue-specific cDC clonality.
- The methodology supports further research into immune cell dynamics and tissue organization.
