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Peroxidase-like phosphate hydrate nanosheets bio-synthesized by a marine Shewanella algae strain for highly sensitive
Jing Yang1, Xiaofan Zhai2, Xucheng Dong3
1College of Marine Life Sciences, Ocean University of China, No. 5 Yushan Road, Qingdao 266003, PR China; CAS Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao 266071, PR China; Open Studio for Marine Corrosion and Protection, Qingdao National Laboratory for Marine Science and Technology, No. 1 Wenhai Road, Qingdao 266235, PR China.
Abstract:
The sensitive and rapid detection of dopamine (DA) is of great significance for early diagnosis of related diseases. Current detection strategies of DA are time-consuming, expensive and inaccurate, while biosynthetic nanomaterials were considered highly stable and environment friendly, which were promising on colorimetric sensing. Thus, in this study, novel zinc phosphate hydrate nanosheets (SA@ZnPNS) biosynthesized by Shewanella algae were designed for the detection of DA. SA@ZnPNS showed high peroxidase-like activity which catalyzed the oxidation reaction of 3,3',5,5'-tetramethylbenzidine in the presence of H2O2. Results showed that the catalytic reaction of SA@ZnPNS followed Michaelis-Menton kinetics, and catalytic process conformed to ping-pong mechanism with chief active species of hydroxyl radicals. The colorimetric detection of DA in human serum samples was performed based on SA@ZnPNS peroxidase-like activity. The linear range of DA detection was 0.1-40 μM, and the detection limit was 0.083 μM. This study provided a simple and practical method for the detection of DA and expanded the application of biosynthesized nanoparticles to biosensing fields.
