Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Embryonic Stem Cells00:58

Embryonic Stem Cells

28.9K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
28.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Risk stratification and relapse pattern in triple-negative breast cancer with pathological complete response after neoadjuvant treatment: the European GAMBIT real-world study.

Nature communications·2026
Same author

Hallmarks of epithelial-mesenchymal plasticity in cancer.

Molecular cancer·2026
Same author

Technical feasibility of a long read, fourth generation sequencing platform in diagnostic profiling of clinical routine samples: a proof-of-concept study.

Pathologica·2026
Same author

Preclinical Rationale, Clinical Efficacy, and Safety of the Selective AKT Kinase Inhibitor Capivasertib in Metastatic Hormone Receptor-Positive, Human Epidermal Growth Factor Receptor 2-Negative Breast Carcinoma: A Practical Narrative Review.

Current oncology (Toronto, Ont.)·2026
Same author

multiDEGGs: Single or Multiomic Differential Network Analysis for Biomarker Discovery and Feature Engineering for Predictive Modeling.

Computational and structural biotechnology journal·2026
Same author

Humic substances enhance the anti-cancer efficacy of standard therapies.

Cell death discovery·2026
Same journal

Sub1 contributes to heart failure with preserved ejection fraction driven by aging in mice.

Nature communications·2026
Same journal

The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells.

Nature communications·2026
Same journal

Signaling downstream of tumor-stroma interaction regulates mucinous colorectal adenocarcinoma apicobasal polarity.

Nature communications·2026
Same journal

Click-polymerized polyenamine membranes for efficient lithium extraction.

Nature communications·2026
Same journal

Joint trajectories of brain atrophy, white matter hyperintensities and cognition quantify brain maintenance.

Nature communications·2026
Same journal

Proton shuttling at electrochemical interfaces under alkaline hydrogen evolution.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Aug 7, 2025

An Ex vivo Culture System to Study Thyroid Development
08:33

An Ex vivo Culture System to Study Thyroid Development

Published on: June 6, 2014

11.6K

Recapitulating thyroid cancer histotypes through engineering embryonic stem cells.

Veronica Veschi1, Alice Turdo2, Chiara Modica1

  • 1Department of Surgical Oncological and Stomatological Sciences, University of Palermo, Palermo, Italy.

Nature Communications
|March 11, 2023
PubMed
Summary
This summary is machine-generated.

Researchers identified the origin of thyroid cancer (TC) by engineering human embryonic stem cell-derived thyroid progenitor cells (TPCs). Specific mutations in TPCs created various TC types, revealing TPCs as the cell of origin for thyroid malignancies.

More Related Videos

An Orthotopic Mouse Model of Anaplastic Thyroid Carcinoma
07:01

An Orthotopic Mouse Model of Anaplastic Thyroid Carcinoma

Published on: April 17, 2013

21.2K
Spontaneous Murine Model of Anaplastic Thyroid Cancer
05:39

Spontaneous Murine Model of Anaplastic Thyroid Cancer

Published on: February 3, 2023

1.7K

Related Experiment Videos

Last Updated: Aug 7, 2025

An Ex vivo Culture System to Study Thyroid Development
08:33

An Ex vivo Culture System to Study Thyroid Development

Published on: June 6, 2014

11.6K
An Orthotopic Mouse Model of Anaplastic Thyroid Carcinoma
07:01

An Orthotopic Mouse Model of Anaplastic Thyroid Carcinoma

Published on: April 17, 2013

21.2K
Spontaneous Murine Model of Anaplastic Thyroid Cancer
05:39

Spontaneous Murine Model of Anaplastic Thyroid Cancer

Published on: February 3, 2023

1.7K

Area of Science:

  • Endocrinology
  • Cancer Biology
  • Stem Cell Biology

Background:

  • Thyroid carcinoma (TC) is the most common endocrine malignancy, but its cell of origin remains unknown.
  • Human embryonic stem cells (hESCs) can differentiate into thyroid progenitor cells (TPCs) and mature thyrocytes in vitro.
  • Mature thyrocytes exhibit limited tumorigenic potential.

Purpose of the Study:

  • To identify the cell of origin for different histotypes of thyroid carcinoma.
  • To investigate the role of specific genetic mutations in TC development.
  • To explore potential therapeutic targets for undifferentiated TC.

Main Methods:

  • CRISPR-Cas9 gene editing was used to introduce specific mutations (BRAFV600E, NRASQ61R, TP53R248Q) into hESC-derived TPCs.
  • Engineered TPCs were analyzed for their capacity to form different TC histotypes.
  • The role of the TIMP1/MMP9/CD44 complex and KISS1R in TC initiation and progression was investigated.

Main Results:

  • TPCs harboring BRAFV600E or NRASQ61R mutations generated papillary or follicular TC, respectively.
  • Addition of TP53R248Q to these mutations resulted in undifferentiated TCs.
  • The TIMP1/MMP9/CD44 complex, in conjunction with KISS1R, is implicated in TC initiation and progression.
  • The same mutations induced teratocarcinomas when introduced in early differentiating hESCs, highlighting the importance of cell type.

Conclusions:

  • hESC-derived TPCs are the cell of origin for follicular cell-derived thyroid carcinomas.
  • Specific genomic alterations in TPCs dictate TC histotype and differentiation status.
  • Targeting KISS1R and TIMP1, alongside increasing radioiodine uptake, may offer therapeutic strategies for undifferentiated TCs.