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Related Experiment Video

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Characterization of Aquatic Biofilms with Flow Cytometry
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Microfluidic Impedance Cytometry for Single-Cell Particulate Inorganic Carbon:Particulate Organic Carbon Measurements

Douwe S de Bruijn1, Dedmer B Van de Waal2, Nico R Helmsing2

  • 1BIOS Lab-on-a-Chip group MESA+ Institute for Nanotechnology Max Planck-University of Twente Center for Complex Fluid Dynamics University of Twente Drienerlolaan 5 Enschede Overijssel 7522 NB The Netherlands.

Global Challenges (Hoboken, NJ)
|March 13, 2023
PubMed
Summary

A new microfluidic impedance cytometer non-invasively measures calcification in coccolithophores. This tool aids understanding of the oceanic carbon cycle by quantifying particulate inorganic carbon (PIC) to particulate organic carbon (POC) ratios.

Keywords:
PIC:POC ratiocalcifying algaemicrofluidic impedance cytometryocean carbon cyclesingle‐cell characterization

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Area of Science:

  • Marine biology
  • Oceanography
  • Biogeochemistry

Background:

  • Coccolithophores are crucial to the oceanic carbon cycle, influencing CO2 flux through photosynthesis and calcification.
  • Quantifying the ratio of particulate inorganic carbon (PIC) to particulate organic carbon (POC) is key to understanding these processes.

Purpose of the Study:

  • To develop a non-invasive, high-throughput method for assessing the calcification state of single coccolithophore cells.
  • To correlate microfluidic impedance measurements with the PIC:POC ratio.

Main Methods:

  • A microfluidic impedance cytometer was used to measure the electrical phase of individual coccolithophore cells.
  • The PIC:POC ratio was determined for the coccolithophore Emiliania huxleyi 920/9 under varying conditions.

Main Results:

  • A strong linear correlation (R² = 0.98) was found between the average electrical phase and the PIC:POC ratio.
  • Acidification showed a clear effect on the PIC:POC ratio, while CO2 treatments were inconclusive for this strain.
  • Lower PIC:POC ratios were observed at higher cell densities.

Conclusions:

  • The microfluidic impedance cytometer is a viable tool for high-throughput PIC:POC ratio quantification.
  • This technology supports future marine carbon cycle projections by enabling analysis under various environmental stressors.