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On RATs and POCT RT-PCRs for COVID and 'flu'

Priscilla Robinson1, Steen Rees2

  • 1School of Psychology and Public Health, La Trobe University, Australia; Australian and New Zealand Journal of Public Health, Australia.

Australian and New Zealand Journal of Public Health
|March 13, 2023
PubMed
Abstract

No abstract available in PubMed .

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...