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MicroRNA-196a-5p targeting LRP1B modulates phenotype of thyroid carcinoma cells
Yaojie Hu1, Chunying Zhang1, Qing Chang1
1Department of Head and Neck Surgery, Tangshan Gongren Hospital, Tangshan, Hebei, China.
Endokrynologia Polska
|March 14, 2023
Summary
MicroRNA-196a-5p is upregulated in thyroid cancer (TC) and promotes tumor progression by targeting LRP1B. Silencing this microRNA inhibits TC cell proliferation, invasion, and migration.
Area of Science:
- Endocrinology
- Molecular Biology
- Oncology
Background:
- Thyroid cancer (TC) is a prevalent endocrine malignancy.
- MicroRNAs (miRNAs) are increasingly recognized for their role in cancer progression.
- The specific role of miRNAs in thyroid diseases requires further elucidation.
Purpose of the Study:
- To investigate the regulatory mechanism of microRNA-196a-5p in thyroid cancer progression.
- To determine if microRNA-196a-5p targets low-density lipoprotein receptor-associated protein 1B (LRP1B) in TC cells.
Main Methods:
- Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and western blot to assess expression levels of microRNA-196a-5p and LRP1B.
- Dual-luciferase reporter assay to confirm the targeting relationship.
- Cell proliferation, invasion, and migration assays (CCK-8, scratch healing, Transwell) to evaluate functional effects.
Main Results:
- MicroRNA-196a-5p expression was significantly upregulated in TC cells, while LRP1B expression was downregulated.
- Upregulation of microRNA-196a-5p promoted TC cell proliferation, invasion, and migration.
- Silencing microRNA-196a-5p reversed these effects, indicating its oncogenic role.
- Dual-luciferase reporter assay confirmed that microRNA-196a-5p directly targets LRP1B.
Conclusions:
- MicroRNA-196a-5p acts as a cancer-promoting microRNA in thyroid cancer.
- The microRNA-196a-5p/LRP1B axis is a key regulatory pathway in TC progression.
- MicroRNA-196a-5p represents a potential therapeutic target for thyroid cancer.
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