Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nucleosomal repeat length in active and inactive genes.

A De Ambrosis1, N Ferrari, S Bonassi

  • 1Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.

FEBS Letters
|December 10, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Agricultural exposures and DNA damage in PBMC of female farmers measured using the alkaline comet assay.

International archives of occupational and environmental health·2024
Same author

Wildlife health surveillance: gaps, needs and opportunities.

Revue scientifique et technique (International Office of Epizootics)·2023
Same author

One year of surgical mask testing at the University of Bologna labs: Lessons learned from data analysis.

Separation and purification technology·2022
Same author

Ready-to-eat salads and berry fruits purchased in Italy contaminated by Cryptosporidium spp., Giardia duodenalis, and Entamoeba histolytica.

International journal of food microbiology·2022
Same author

Cytokines and parturition: investigating adiponectin, TNF-α, and IL-6 in mother-newborn pairs.

The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·2022
Same author

Cost-effectiveness of a family-based multicomponent outpatient intervention program for children with obesity in Germany.

Public health·2020
Same journal

Investigating transcription factor dynamics in health and disease using FRAP.

FEBS letters·2026
Same journal

Regulation of CFTR stability at the plasma membrane-Mechanisms and therapeutic opportunities in cystic fibrosis.

FEBS letters·2026
Same journal

Identification of a Shiga toxin A-derived peptide internalized into Gb3 receptor-bearing cells via interaction with the Shiga toxin B subunit.

FEBS letters·2026
Same journal

The dual role of lectins in cancer-immunotherapy tools and therapeutic targets.

FEBS letters·2026
Same journal

Decoding the dynamic extracellular matrix in cancer-3D models and bioscaffolds rewire the rules of tumor progression.

FEBS letters·2026
Same journal

Extending the classical sequence-structure-function paradigm through protein dynamics and context-dependent behavior.

FEBS letters·2026
See all related articles

Chromatin structure varies between active and inactive genes, with distinct nucleosomal spacing observed even in metaphase chromosomes. These findings suggest dynamic DNA-histone interactions facilitate chromatin remodeling for gene regulation.

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Genetics

Background:

  • Chromatin organization is crucial for gene regulation.
  • Nucleosomal repeat length (NRL) is a key parameter of chromatin structure.
  • Understanding variations in NRL is essential for deciphering gene accessibility.

Purpose of the Study:

  • To investigate differences in nucleosomal repeat lengths between active and inactive chromatin.
  • To determine if these differences persist in metaphase chromosomes.
  • To explore the role of DNA-core histone interactions in chromatin remodeling.

Main Methods:

  • Measurement of nucleosomal repeat lengths in HeLa cells.
  • Analysis of total chromatin, H4 histone, and beta-DR gene regions.
  • Comparison of NRL in logarithmically growing cells and metaphase chromosomes.

Related Experiment Videos

Main Results:

  • Significant differences in nucleosomal spacing were found between inactive and actively transcribing chromatin.
  • These spacing differences were maintained in metaphase chromosomes, despite a general shortening of NRL.
  • Both active and inactive chromatin showed a reduced NRL during metaphase.

Conclusions:

  • Nucleosomal spacing is dynamically regulated and differs between active and inactive chromatin.
  • Dynamic alterations in DNA-core histone interactions likely underlie chromatin remodeling.
  • These findings support models of transient modifications in chromatin organization for regulatory purposes.