Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Polyclonal selection of immune checkpoint mutations in thyroid autoimmunity.

Nature·2026

Same author

A chromosome-level genome assembly of the Hispid cotton rat (Sigmodon hispidus), a model for human pathogenic virus infections.

BMC biology·2025

Same author

Hi-C scaffolded short- and long-read genome assemblies of the California sea lion are broadly consistent for syntenic inference across 45 million years of evolution.

Molecular ecology resources·2021

Same author

Simvastatin enhances the efficacy of nilotinib in chronic myeloid leukaemia by post-translational modification and drug transporter modulation.

Anti-cancer drugs·2021

Same author

Single-cell atlas of the first intra-mammalian developmental stage of the human parasite Schistosoma mansoni.

Nature communications·2020

Same author

Chromosome-level genome assembly for giant panda provides novel insights into Carnivora chromosome evolution.

Genome biology·2019

Same journal

A Novel Laboratorial Approach to Evaluate Bacterial Microleakage of Endodontic Sealers.

Current protocols·2026

Same journal

TRIAGE Toolkit: Streamlined Discovery of Regulatory Genes and Elements.

Current protocols·2026

Same journal

High-throughput Profiling of Pseudouridines in Microbiome-derived Bacterial RNA.

Current protocols·2026

Same journal

Recombinant Protein Expression in Rhodococcus species.

Current protocols·2026

Same journal

Streamlined In Vitro Transcription for Generating Self-Amplifying RNA With Modified Nucleotides.

Current protocols·2026

Same journal

CODEC Library Preparation From Genomic DNA.

Current protocols·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Aug 6, 2025

Flow Cytometry Purification of Mouse Meiotic Cells

Flow Cytometry Purification of Mouse Meiotic Cells

Published on: April 15, 2011

Chromosome Analysis and Sorting Using Conventional Flow Cytometers.

¹Wellcome Sanger Institute, Cytometry Core Facility, Hinxton, Cambridge, United Kingdom.

Current Protocols

|March 15, 2023

Summary

This summary is machine-generated.

This study introduces a new method for chromosome analysis using DAPI and propidium iodide (PI) dyes. This technique allows bivariate flow karyotyping on standard flow cytometers, simplifying chromosome identification.

Keywords:

DAPI chromosomes flow karyotyping propidium iodide

More Related Videos

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Published on: March 24, 2023

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Published on: July 12, 2018

Related Experiment Videos

Last Updated: Aug 6, 2025

Flow Cytometry Purification of Mouse Meiotic Cells

Flow Cytometry Purification of Mouse Meiotic Cells

Published on: April 15, 2011

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Published on: March 24, 2023

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Published on: July 12, 2018

Area of Science:

Genetics
Molecular Biology
Cytometry

Background:

Bivariate flow karyotyping is crucial for distinguishing chromosomes based on DNA content and base composition.
Traditional methods use Hoechst (HO) and Chromomycin A3 (CA3) dyes, requiring specialized, high-power flow cytometers.
This limitation restricts accessibility for many research laboratories.

Purpose of the Study:

To present an alternative chromosome staining technique for bivariate flow karyotyping.
To enable chromosome analysis using conventional flow cytometers with standard laser configurations.
To provide a more accessible method for chromosome identification and analysis.

Main Methods:

Developed a chromosome staining protocol using DAPI and propidium iodide (PI).
Utilized standard cell culture and metaphase harvest techniques for suspension and adherent cell lines.
Employed polyamine isolation buffer for chromosome suspension preparation.
Performed bivariate flow karyotyping using both cell analyzers and cell sorters.
Included protocols for purification of flow-sorted chromosomes.

Main Results:

Successfully stained chromosomes with DAPI and PI, enabling analysis on conventional flow cytometers.
Demonstrated the feasibility of bivariate flow karyotyping with this dye combination on standard equipment.
Provided detailed protocols for cell preparation, staining, and flow cytometry analysis.

Conclusions:

The DAPI/PI staining method offers a viable and accessible alternative for bivariate flow karyotyping.
This technique overcomes the need for specialized equipment, broadening the application of flow karyotyping.
Facilitates chromosome analysis and purification for various genetic and molecular biology applications.