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Related Experiment Video

Updated: Aug 6, 2025

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

Pseudotyped Viruses for Mammarenavirus.

Qianqian Li¹, Weijing Huang², Youchun Wang^3,4

¹Jiangsu Recbio Technology Co., Ltd., Taizhou, China.

Advances in Experimental Medicine and Biology

|March 15, 2023

Summary

This summary is machine-generated.

Pseudotyped mammarenaviruses, created using various viral vectors, are essential tools for studying these rodent-borne viruses. They aid in understanding viral infections, developing diagnostics, and screening treatments for diseases like Lassa fever.

Keywords:

Arenaviruses Lassa virus Lentiviral vectors Lymphocytic choriomeningitis virus MLV-based vectors Mammarenavirus Pseudotyped viruses VSV-based vectors

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Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses

Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses

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Last Updated: Aug 6, 2025

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

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Published on: March 1, 2019

Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses

Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses

Published on: January 15, 2020

Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses

Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses

Published on: November 21, 2023

Area of Science:

Virology
Infectious Diseases
Molecular Biology

Background:

Mammarenaviruses, including Old World (OW) and New World (NW) types, are rodent-borne viruses causing severe infections and hemorrhagic fevers.
The Old World (OW) arenaviruses encompass lymphocytic choriomeningitis virus (LCMV) and the lethal Lassa virus (LASV).
Rodent transmission to humans results in significant acute illness.

Purpose of the Study:

To highlight the utility of pseudotyped mammarenaviruses as research tools.
To outline the diverse applications of pseudotyped mammarenavirus systems.
To summarize the methods used in constructing these pseudotyped viruses.

Main Methods:

Construction of pseudotyped mammarenaviruses utilizing lentiviral vectors (HIV-1, SIV, FIV-based).
Application of other viral vector systems, including VSV-based and MLV-based vectors.
Employment of reverse genetic approaches for pseudotyped virus generation.

Main Results:

Pseudotyped mammarenaviruses facilitate research across multiple domains.
These systems are instrumental in receptor binding and neutralizing antibody studies.
They enable inhibitor screening and analysis of viral pathogenesis.

Conclusions:

Pseudotyped mammarenaviruses are versatile tools for studying mammarenavirus biology.
Their applications span receptor research, diagnostics, and therapeutic development.
These systems are crucial for advancing our understanding of viral infection mechanisms, including endocytosis and fusion.