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Fluorescence Quantum Yield Standards for the UV/Visible/NIR: Development, Traceable Characterization, and

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This study introduces 12 new fluorescence quantum yield (Φf) standards for reliable photometer calibration. These validated standards improve the accuracy and comparability of fluorescence quantum yield measurements across different labs and instruments.

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Area of Science:

  • Spectroscopy
  • Materials Science
  • Analytical Chemistry

Background:

  • Accurate fluorescence quantum yield (Φf) determination is crucial for developing new molecular and nanoscale reporters.
  • Reliable Φf measurements depend on validated instrument calibration and well-characterized standards.
  • Existing standards introduce uncertainty due to variations in their certified Φf values.

Purpose of the Study:

  • To develop and certify a novel set of fluorescence quantum yield standards.
  • To provide reference materials for accurate and comparable Φf measurements in the 330-1000 nm range.
  • To enhance the reliability of spectroscopic characterization for new emitters.

Main Methods:

  • Absolute determination of Φf values using two independently calibrated integrating sphere setups.
  • Development of 12 new fluorescence quantum yield standards covering the 330-1000 nm wavelength region.
  • Certification procedure including homogeneity, stability studies, and uncertainty budget calculations.

Main Results:

  • A set of 12 novel fluorescence quantum yield standards with certified Φf values has been developed.
  • The standards cover a broad spectral range from 330 nm to 1000 nm.
  • Detailed characterization and uncertainty budgets ensure the reliability of the certified Φf values.

Conclusions:

  • The new fluorescence quantum yield standards provide a reliable basis for improved comparability of Φf data.
  • These reference materials are essential for the rational design and characterization of next-generation molecular and nanoscale reporters.
  • The availability of these certified standards will advance the field of fluorescence spectroscopy.