Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hormones of the Adrenal Glands01:31

Hormones of the Adrenal Glands

2.7K
Adrenal hormones play a pivotal role in maintaining the body's electrolyte balance and orchestrating responses to stress, showcasing the intricate functions of the adrenal cortex and medulla.
The adrenal cortex, a powerhouse of hormone synthesis, generates over two dozen corticosteroid hormones. The zona glomerulosa produces mineralocorticoids, exemplified by aldosterone, influencing the electrolyte composition of body fluids. The synthesis of glucocorticoids such as cortisol and...
2.7K
Antiasthma Drugs: Inhaled Corticosteroids and Glucocorticoids01:25

Antiasthma Drugs: Inhaled Corticosteroids and Glucocorticoids

335
Inhaled corticosteroids (ICS) are anti-inflammatory drugs used primarily in treating persistent asthma and providing long-term maintenance. They target the bronchial mucosa, the lining of the airways, to control inflammation, a critical factor in asthma progression and exacerbation.
ICS work through a multifaceted mechanism of action. They suppress the inflammatory response caused by the proliferation of TH cells. They also reduce the transcription of the IL-2 gene, which is involved in the...
335
Adrenal Gland Disorders01:27

Adrenal Gland Disorders

1.7K
Adrenal gland disorders manifest when the production of adrenal hormones deviates from the norm, resulting in either excessive or insufficient concentrations.
Adrenal insufficiency, characterized by insufficient cortisol and aldosterone production, leads to conditions like Addison's disease. This disorder, affecting the adrenal cortex, exhibits symptoms such as skin bronzing, dehydration, low blood pressure, fatigue, and weight loss. Congenital adrenal hyperplasia, a genetic ailment causing...
1.7K
Hypothalamic-Pituitary Axis01:37

Hypothalamic-Pituitary Axis

60.9K
The response to stress—be it physical or psychological, acute or chronic—involves activation of the Hypothalamic-Pituitary-Adrenal (HPA) axis. The HPA axis is part of the neuroendocrine system because it involves both neuronal and hormonal communication. Its function is to regulate homeostatic systems—metabolic, cardiovascular, and immune—providing the necessary means to respond to a stressor.
60.9K
Gonadal and Placental Hormones01:24

Gonadal and Placental Hormones

1.6K
The gonads, namely the testes in males and the ovaries in females, are pivotal in producing gonadal hormones that orchestrate the intricate processes of sexual development and reproduction.
In males, testosterone is the primary gonadal androgen. It plays a central role in the maturation of male reproductive organs — the penis and testes. Additionally, testosterone is instrumental in the development of secondary sexual characteristics — a deep voice as well as facial and pubic hair...
1.6K
Testosterone: Functions and Regulation01:26

Testosterone: Functions and Regulation

876
The intricate hormonal interplay essential for male reproductive health begins with the release of gonadotropin-releasing hormone (GnRH) by the hypothalamus. This hormone prompts the pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). LH targets the Leydig cells in the testes, stimulating them to produce and release testosterone. In concert with testosterone, FSH acts on the Sertoli cells within the seminiferous tubules to facilitate the release of...
876

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Atrial natriuretic peptide counteracts aldosterone secretion by preventing acute angiotensin II-induced cAMP signalling.

British journal of pharmacology·2026
Same author

Association Between Papillary Thyroid Cancer and Primary Aldosteronism in Individuals With Hypertension.

The Journal of clinical endocrinology and metabolism·2024
Same author

Dlk1 is a novel adrenocortical stem/progenitor cell marker that predicts malignancy in adrenocortical carcinoma.

bioRxiv : the preprint server for biology·2024
Same author

An in Vitro triple screen model for human mineralocorticoid receptor activity.

The Journal of steroid biochemistry and molecular biology·2024
Same author

Familial hyperaldosteronism: an European Reference Network on Rare Endocrine Conditions clinical practice guideline.

European journal of endocrinology·2024
Same author

Investigating the cut-off values of captopril challenge test for primary aldosteronism using the novel chemiluminescent enzyme immunoassay method: a retrospective cohort study.

Hypertension research : official journal of the Japanese Society of Hypertension·2024
Same journal

Androgen receptor as therapeutic indicator for LAR-positive triple-negative breast cancer: Preclinical and clinical assessment.

The Journal of steroid biochemistry and molecular biology·2026
Same journal

The 24,25(OH)<sub>2</sub>D<sub>3</sub>-TLCD3B signaling complex and reproductive function in mice and men.

The Journal of steroid biochemistry and molecular biology·2026
Same journal

Testosterone and epitestosterone differentially regulate UDP-glucuronosyltransferases (UGT) activity.

The Journal of steroid biochemistry and molecular biology·2026
Same journal

Interferon-gamma-inducible protein-10 is associated to increased primary bile acids and fibrosis in primary sclerosing cholangitis.

The Journal of steroid biochemistry and molecular biology·2026
Same journal

Testosterone increases clonogenicity and self-renewal capacity of human glioblastoma cells.

The Journal of steroid biochemistry and molecular biology·2026
Same journal

Role of 5α-reductase inhibitors in Parkinson's disease: Gambling or not?

The Journal of steroid biochemistry and molecular biology·2026
See all related articles

Related Experiment Video

Updated: Aug 6, 2025

Cortisol Measurement in Koala Phascolarctos cinereus Fur
09:11

Cortisol Measurement in Koala Phascolarctos cinereus Fur

Published on: August 23, 2019

10.8K

18-Oxocortisol: A journey.

Celso E Gomez-Sanchez1, Elise P Gomez-Sanchez2

  • 1Research Service, G. V. (Sonny) Montgomery VA Medical Center, Jackson, MS, USA; Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, MS, USA.

The Journal of Steroid Biochemistry and Molecular Biology
|March 15, 2023
PubMed
Summary
This summary is machine-generated.

Researchers discovered 18-oxocortisol, a weak mineralocorticoid, which helps distinguish primary aldosteronism subtypes. Elevated levels indicate aldosterone-producing adenomas and can predict specific gene mutations like KCNJ5.

Keywords:
18-hydroxycortisol18-oxocortisol, CYP11B2AldosteroneCyp17a1Zona Glomerulosa

More Related Videos

Extraction and Analysis of Cortisol from Human and Monkey Hair
08:38

Extraction and Analysis of Cortisol from Human and Monkey Hair

Published on: January 24, 2014

28.5K
Bovine Ovarian Cortex Tissue Culture
06:32

Bovine Ovarian Cortex Tissue Culture

Published on: January 14, 2021

4.8K

Related Experiment Videos

Last Updated: Aug 6, 2025

Cortisol Measurement in Koala Phascolarctos cinereus Fur
09:11

Cortisol Measurement in Koala Phascolarctos cinereus Fur

Published on: August 23, 2019

10.8K
Extraction and Analysis of Cortisol from Human and Monkey Hair
08:38

Extraction and Analysis of Cortisol from Human and Monkey Hair

Published on: January 24, 2014

28.5K
Bovine Ovarian Cortex Tissue Culture
06:32

Bovine Ovarian Cortex Tissue Culture

Published on: January 14, 2021

4.8K

Area of Science:

  • Endocrinology
  • Steroid Biochemistry
  • Hypertension Research

Background:

  • Investigating mineralocorticoids for low renin hypertension cases.
  • Isolation of 18-hydroxycortisol from human urine.
  • Exploration of steroid precursors for aldosterone synthesis.

Purpose of the Study:

  • Discover novel mineralocorticoids.
  • Characterize the newly found steroid 18-oxocortisol.
  • Determine the clinical utility of 18-oxocortisol measurements in primary aldosteronism.

Main Methods:

  • Incubation of bullfrog adrenals with cortisol.
  • Structural elucidation of 18-oxocortisol.
  • Measurement of 18-oxocortisol levels in patient subtypes.

Main Results:

  • Discovery of 18-oxocortisol, a weak mineralocorticoid.
  • Identification of its synthesis in the zona glomerulosa with co-expression of CYP11B2 and CYP17A1.
  • Significant elevation of 18-oxocortisol in aldosterone-producing adenomas compared to idiopathic bilateral hyperaldosteronism.
  • Correlation of higher 18-oxocortisol levels with KCNJ5 mutations in adenomas.

Conclusions:

  • 18-Oxocortisol is a clinically valuable biomarker for differentiating primary aldosteronism subtypes.
  • Measurement of 18-oxocortisol aids in predicting somatic mutations within aldosterone-producing adenomas.