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BAFF induces CXCR5 expression during B cell differentiation in bone marrow.

Hajime Koizumi1, Wataru Fujii2, Chizu Sanjoba1

  • 1Laboratory of Molecular Immunology, Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.

Biochemistry and Biophysics Reports
|March 17, 2023
PubMed
Summary

B cell activating factor (BAFF) directly increases CXCR5 expression on bone marrow B cells, promoting their differentiation and migration to secondary lymphoid organs. This study clarifies BAFF's role in primary B cell development.

Keywords:
B cell developmentBAFFBone marrowCXCR5

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Area of Science:

  • Immunology
  • Cell Biology
  • Developmental Biology

Background:

  • B cell activating factor (BAFF) is crucial for B cell maturation in secondary lymphoid organs.
  • The function of BAFF in bone marrow B cell development remains incompletely understood.

Purpose of the Study:

  • To investigate the role of BAFF in B cell development within the bone marrow.
  • To determine if BAFF directly influences B cell differentiation markers in the bone marrow.

Main Methods:

  • Utilized BAFF-deficient (BAFF-KO) mice to examine B cell development.
  • Analyzed mRNA and protein expression of B cell differentiation markers, including CXCR5.
  • Conducted in vitro studies culturing BAFF-KO bone marrow cells with BAFF.

Main Results:

  • BAFF deficiency led to reduced CXCR5 mRNA and protein expression on bone marrow IgM+ cells.
  • In vitro BAFF treatment increased CXCR5 expression on IgM+ cells from BAFF-KO mice.
  • BAFF synergized with TNF-α and IL-6 to upregulate CXCR5 expression on B-lineage cells.

Conclusions:

  • BAFF directly induces CXCR5 expression on B-lineage cells in the bone marrow.
  • This BAFF-mediated CXCR5 upregulation is critical for B cell differentiation and migration.
  • BAFF plays a significant role in B cell development within the primary lymphoid organ.