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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
Published on: March 17, 2023
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Multiparametric Biosensors for Characterizing Extracellular Vesicle Subpopulations.
Narges Shaabani1, Sabrina Rodrigues Meira1,2, Marcelo Marcet-Palacios3
1Nanotechnology Research Centre, National Research Council Canada, Edmonton, Alberta T6G 2M9, Canada.
ACS Pharmacology & Translational Science
|March 17, 2023
Summary
Recent advances in exosome isolation and sensing technologies, including microfluidics and advanced biosensors, are improving the characterization of these crucial intercellular communication vesicles for therapeutic applications.
Area of Science:
- Biotechnology
- Nanomedicine
- Cell Biology
Background:
- Extracellular vesicles (EVs), particularly exosomes, are key for intercellular communication and hold promise for precision therapy.
- Characterizing and isolating exosomes (30-150 nm) is challenging due to their small size and conventional method limitations.
Purpose of the Study:
- To review recent advancements in exosome isolation, purification, and sensing technologies.
- To discuss challenges in exosome size heterogeneity and the application of biosensors.
- To explore advanced sensing platforms for exosome detection and ultrastructure analysis.
Main Methods:
- Microfluidics
- Acoustic technologies
- Size exclusion chromatography
- Colorimetric, fluorescent, electronic, SPR, and Raman spectroscopy
- Cryogenic electron tomography and microscopy
Main Results:
- Novel platforms enhance exosome isolation and purification efficiency.
- Biosensor technology offers new avenues for exosome characterization and detection.
- Advanced imaging techniques provide insights into exosome ultrastructure.
Conclusions:
- Technological advancements are crucial for overcoming exosome research challenges.
- Future needs include integrated multiparametric systems for comprehensive exosome analysis.
- These technologies will drive progress in exosome-based precision medicine and drug delivery.
