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Related Experiment Video

Updated: Aug 6, 2025

Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence
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Detection and Isolation of Circulating Tumor Cells from Breast Cancer Patients Using CUB Domain-Containing Protein 1.

Kai Bartkowiak1, Parinaz Mossahebi Mohammadi1, Sebastian Gärtner1

  • 1Department of Tumour Biology, University Medical Centre Hamburg-Eppendorf, Martinistraße 52, 20246 Hamburg, Germany.

Journal of Proteome Research
|March 17, 2023
PubMed
Summary

Researchers identified CUB domain-containing protein 1 (CDCP1) as a biomarker for isolating mesenchymal circulating tumor cells (CTCs) and disseminated tumor cells (DTCs). This new method aids in capturing EpCam-negative CTCs, crucial for understanding cancer metastasis.

Keywords:
CDCP1EGFRbone marrowbreast cancercirculating tumor cells

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Area of Science:

  • Oncology
  • Biomarker Discovery
  • Cell Biology

Background:

  • Cancer metastasis involves circulating tumor cells (CTCs) and disseminated tumor cells (DTCs).
  • Isolating mesenchymal CTCs/DTCs is challenging due to lack of specific biomarkers.
  • Mesenchymal cells often downregulate epithelial markers like EpCam.

Purpose of the Study:

  • To develop a method for isolating mesenchymal CTCs and DTCs.
  • To identify novel biomarkers for these challenging-to-isolate cells.
  • To improve detection of CTCs in breast cancer patients, especially those with poor prognosis.

Main Methods:

  • Analyzed breast cancer cell line BC-M1 using stable isotope labeling by amino acids in cell culture (SILAC) and mass spectrometry.
  • Investigated expression of CUB domain-containing protein 1 (CDCP1) in cancer cells and peripheral blood mononuclear cells.
  • Developed a magnetic-activated cell sorting (MACS) approach using CDCP1's extracellular domain.

Main Results:

  • Identified high CDCP1 levels in mesenchymal breast cancer cell lines.
  • CDCP1 was virtually absent in peripheral blood mononuclear cells.
  • Confirmed CDCP1-positive CTCs in 8/30 breast cancer patients; MACS enriched EpCam-negative CTCs.

Conclusions:

  • CDCP1 is a promising biomarker for isolating mesenchymal CTCs.
  • The CDCP1-based MACS method effectively enriches EpCam-negative CTCs.
  • This approach is valuable for studying and potentially treating aggressive breast cancers like triple-negative breast cancer.