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Related Experiment Video

Updated: Aug 6, 2025

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation

Published on: October 29, 2013

PEG-fibrin conjugates: the PEG impact on the polymerization dynamics.

Alesia A Bakulina¹, Guzel R Musina², Arsenii A Gavdush²

¹Institute for Regenerative Medicine, Sechenov University, Moscow, Russia. shpichka_a_i@staff.sechenov.ru.

|March 17, 2023

Summary

Polyethylene glycol (PEG) modification of fibrin accelerates gelation, creating softer biomaterials for drug delivery. PEGylated fibrin forms stable, water-shelled aggregates, unlike native fibrin.

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Last Updated: Aug 6, 2025

Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation

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Published on: October 29, 2013

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Published on: December 23, 2013

Area of Science:

Biomaterials Science
Polymer Chemistry
Biophysics

Background:

Fibrin is a promising biomaterial for drug delivery and regenerative medicine.
Limited understanding exists regarding the post-modification processes of fibrin, particularly its conjugates with polyethylene glycol (PEG).

Purpose of the Study:

To investigate the structural and dynamic differences between native fibrin and PEGylated fibrin conjugates.
To characterize the gelation process and the effect of PEGylation on fibrin polymerization dynamics using terahertz (THz) pulsed spectroscopy and rheology.

Main Methods:

Terahertz (THz) pulsed spectroscopy was employed to monitor polymerization dynamics.
Rheology was used to assess the mechanical properties and gelation kinetics.
Comparative analysis of native fibrin and fibrin modified with varying PEG ratios (5:1 and 10:1).

Main Results:

PEGylated fibrin exhibited a homogenously soft surface compared to native fibrin.
PEGylation significantly decreased gelation time: from 42.75 min (native) to 31.26 min (5:1 PEG) and 35.09 min (10:1 PEG).
THz spectroscopy indicated rapid polymerization in PEGylated fibrin, preventing calculation of the time constant, unlike native fibrin (14.4 ± 2.8 min).

Conclusions:

PEG-fibrin conjugates form homogenous, water-shelled aggregates without bundling, leading to faster gelation and system stabilization.
The study highlights the utility of THz spectroscopy and rheology in characterizing fibrin gelation and PEGylation effects.
Modified fibrin presents a tunable biomaterial with enhanced properties for advanced applications.