A mesh microelectrode array for non-invasive electrophysiology within neural organoids
Matthew McDonald1, David Sebinger2, Lisa Brauns1
1NMI Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770, Reutlingen, Germany; Max Planck Institute for Molecular Biomedicine, Röntgenstr. 20, 48149, Münster, Germany.
Biosensors & Bioelectronics
|March 17, 2023
Summary
Researchers developed novel mesh microelectrode arrays for culturing and recording from neural organoids. These suspended hammock-like devices allow for long-term organoid growth and capture electrical activity within the 3D tissue.
Area of Science:
- Neuroscience
- Biomedical Engineering
- Tissue Engineering
Background:
- Organoids are advanced in vitro models of human physiology.
- Evaluating functional activity in neural organoids requires specialized tools.
- Existing microelectrode arrays are not well-suited for 3D organoid structures.
Purpose of the Study:
- To develop novel microelectrode arrays for neural organoids.
- To enable long-term culture and functional recording of organoids.
- To overcome limitations of current electrophysiology techniques for organoids.
Main Methods:
- Fabrication of suspended hammock-like mesh microelectrode arrays.
- Culturing neural organoids on these mesh arrays.
- Demonstration of organoid engulfment and long-term culture (up to 1 year).
- Recording spontaneous electrical activity within the organoid volume.
Main Results:
- Successful growth of neural organoids enveloping the mesh microelectrode arrays.
- Sustained organoid culture on the meshes for up to one year.
- Proof-of-principle recordings of spontaneous neural activity across the organoid.
- Demonstrated compatibility of organoids with mesh electronics.
Conclusions:
- The novel mesh microelectrode arrays support long-term neural organoid culture.
- These arrays enable functional electrophysiological recordings from 3D organoids.
- This technology represents a new class of tools for studying 3D electrically active tissue models.
