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Updated: Aug 6, 2025
Isolation of Atrial Myocytes from Adult Mice
Published on: July 25, 2019
Protocol for inducing cellular ablation in the mouse atrioventricular conduction system
Lin Wang1, Jared Gabriel L Dela Rosa2, Nikhil V Munshi3
1Department of Internal Medicine, Cardiology Division, UT Southwestern Medical Center, Dallas, TX 75390, USA.
Abstract:
Damage to the atrioventricular conduction system (AVCS), the main electrical connection between the atrial and ventricular chambers, can result in a variety of cardiac conduction disorders. Here, we provide a protocol for selective damage of the mouse AVCS to study its response during injury. We describe tamoxifen-induced cellular ablation, detection of AV block through electrocardiography, and quantification of histological and immunofluorescence markers to analyze the AVCS. This protocol can be used to study mechanisms associated with AVCS injury repair and regeneration. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021).1.
Insights
This study introduces a mouse model to selectively damage the atrioventricular conduction system (AVCS), enabling research into cardiac conduction disorders and AVCS repair mechanisms.
Area of Science:
- Cardiology
- Molecular Biology
- Regenerative Medicine
Background:
- Damage to the atrioventricular conduction system (AVCS) can cause cardiac conduction disorders.
- Understanding AVCS injury response is crucial for developing treatments.
Purpose of the Study:
- To provide a protocol for selective AVCS damage in mice.
- To facilitate the study of AVCS response to injury, repair, and regeneration.
Main Methods:
- Tamoxifen-induced cellular ablation to target AVCS cells.
- Electrocardiography to detect atrioventricular block.
- Histological and immunofluorescence analysis to assess AVCS changes.
Main Results:
- A reproducible protocol for inducing and analyzing AVCS damage in mice was established.
- The model allows for detailed investigation of cellular and tissue responses to AVCS injury.
Conclusions:
- This protocol serves as a valuable tool for studying the mechanisms of AVCS injury, repair, and regeneration.
- It enables further research into potential therapeutic strategies for cardiac conduction disorders.
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