Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

13.3K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
13.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dupilumab opens a therapeutic window in food protein-induced enterocolitis syndrome by unlicensing dendritic cells.

The journal of allergy and clinical immunology. Global·2025
Same author

Enhancing allergy diagnosis: mass spectrometry as a complementary technique to the basophil activation test.

Frontiers in allergy·2025
Same author

Safety and Efficacy of Intravenous Immune Globulin 10% (BIVIGAM<sup>®</sup>) in Children with Primary Immune Deficiency.

Journal of clinical immunology·2025
Same author

Basophil activation test; User's manual.

Journal of immunological methods·2025
Same author

STAT4 Phosphorylation of T-helper Cells predicts surgical outcomes in Refractory Chronic Rhinosinusitis.

medRxiv : the preprint server for health sciences·2024
Same author

Towards an FDA-cleared basophil activation test.

Frontiers in allergy·2023

Related Experiment Video

Updated: Aug 6, 2025

Basophil Activation Test for Allergy Diagnosis
07:22

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

8.3K

Towards standardizing basophil identification by flow cytometry.

Soren Ulrik Sonder1, Matthew Plassmeyer1, Denise Loizou1

  • 1Amerimmune, McLean, VA, United States.

Frontiers in Allergy
|March 20, 2023
PubMed
Summary
This summary is machine-generated.

Accurate basophil identification using flow cytometry requires at least two surface markers. Combining CD123 with CD193, HLADR-negative, or FcɛRI offers the best results for basophil enumeration and activation testing.

Keywords:
CBC (complete blood count)basophil actication testbasophil activating test (BAT)basophil identification markersflow cytometry

More Related Videos

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
10:22

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Published on: September 16, 2011

33.7K
Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation
12:05

Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation

Published on: November 3, 2018

11.7K

Related Experiment Videos

Last Updated: Aug 6, 2025

Basophil Activation Test for Allergy Diagnosis
07:22

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

8.3K
Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
10:22

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Published on: September 16, 2011

33.7K
Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation
12:05

Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation

Published on: November 3, 2018

11.7K

Area of Science:

  • Immunology
  • Hematology
  • Flow Cytometry

Background:

  • Basophils constitute less than 2% of white blood cells (WBC).
  • Standardized methods for basophil identification via flow cytometry are lacking.
  • Increased demand for basophil activation tests (BAT) necessitates reliable identification techniques.

Purpose of the Study:

  • To evaluate different flow cytometry marker combinations for accurate basophil identification.
  • To compare flow cytometry basophil counts with complete blood count (CBC) results.
  • To assess the impact of stimulation on basophil identification markers.

Main Methods:

  • Whole blood was stained with antibodies against IgE, CD123, CD193, CD203c, CD3, HLADR, FcɛRI, CRTH2, and CD45.
  • Samples were analyzed unstimulated and after stimulation with Anti-IgE and fMLP.
  • Flow cytometry results were compared to CBC from an FDA-approved hematological analyzer.

Main Results:

  • Two-marker identification approaches outperformed single-marker methods for basophil identification.
  • Flow cytometry-derived basophil percentages correlated well with CBC, though CBC values were higher.
  • Basophil activation stimulation did not interfere with the identification markers.

Conclusions:

  • Utilizing two surface markers is recommended for basophil identification in flow cytometry.
  • A third marker can enhance purity if a highly specific basophil fraction is needed.
  • Combinations including CD123 with CD193, HLADR-negative, or FcɛRI demonstrated optimal performance.